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IFNG antibody (A00393-3) performs well in IHC, WB, ELISA (Cap) and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 15 Publications

a00393 3 ifng primary antibodies ihc testing 2

IHC analysis of IFNG using anti-IFNG antibody (A00393-3).
IFNG was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IFNG Antibody (A00393-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-Interferon gamma/IFNG Antibody Picoband®

IFNG antibody

15 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IHC, WB, ELISA (Cap)

Tested Applications

IFNG antibody was positively detected by WB in:

human placenta tissue, rat spleen tissue, mouse spleen tissue, mouse thymus tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

IFNG antibody was positively detected by IHC in:

human tonsil tissue, mouse colon tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Mouse

Tested

Recommend

Monkey

Predicted

Product Information

Product Name

Anti-Interferon gamma/IFNG Antibody Picoband®
View all IFNG Antibodies

Form

Lyophilized

Uniprot

P01579

Gene ID

3458

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Interferon gamma recombinant protein (Position: Q24-G161).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

19 kDa

Calculated Molecular Weight

19.3 kDa

Background of IFNG

IInterferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Cite This Product

Anti-Interferon gamma/IFNG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00393-3)

Protocols

Western Blot protocol for IFNG antibody

Review Protocol

Immunohistochemistry (IHC) protocol for IFNG antibody

Review Protocol

Publications

Immune Imbalance in Th1/Th2 and Th17/Treg as a Contributor to Endometrial Polyps: A Prospective Laboratory-Based Study

Published: 2025-11-30

Journal: Clinical and Experimental Obstetrics & Gynecology

Application: IHC

Impact Factor: 0.60

Species: Human

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Anti-Interferon gamma/IFNG Antibody Picoband®

IFNG antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Immune Imbalance in Th1/Th2 and Th17/Treg as a Contributor to Endometrial Polyps: A Prospective Laboratory-Based Study

Targeting Talin1 to reprogram dendritic cell activation landscapes: A mechanistically grounded cell therapy for multiple sclerosis model

Resveratrol alleviates testicular toxicity induced by anti-PD-1 through regulating the NRF2-SLC7A11-GPX4 pathway

In situ editing of tumour cell membranes induces aggregation and capture of PD-L1 membrane proteins for enhanced cancer immunotherapy

Endoplasmic reticulum stress potentiates the immunosuppressive microenvironment in hepatocellular carcinoma by promoting the release of SNHG6-enriched small extracellular vesicles

A “2 + 2” strategy for tumor immune microenvironment remodeling based on complementary immune checkpoint blockade

A Synbiotic Ameliorates Con A-Induced Autoimmune Hepatitis in Mice through Modulation of Gut Microbiota and Immune Imbalance

Quercetin inhibiting the PD-1/PD-L1 interaction for immune-enhancing cancer chemopreventive agent

Bu-Shen-Fang-Chuan formula attenuates T-lymphocytes recruitment in the lung of rats with COPD through suppressing CXCL9/CXCL10/CXCL11-CXCR3 axis

Bone Marrow-Derived Mesenchymal Stem Cells Modified with Akt1 Ameliorates Acute Liver GVHD

Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats

The expression and significance of IL-6, IFN-γ, SM22α, and MMP-2 in rat model of aortic dissection.

Human interleukin-10 gene inhibits acute rejection by triggering apoptosis in allograft vascular transplantation

Atorvastatin ameliorates experimental autoimmune neuritis by decreased Th1/Th17 cytokines and up-regulated T regulatory cells

Anti-arthritis activity of cationic materials

Review

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