Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine™

Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ifng in cell culture supernatants and serum. Sensitivity: 5pg/ml. Cited in 60 publication(s).

Product Info Summary

SKU: EK0375
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine™

See all IFN-gamma products

SKU/Catalog Number

EK0375

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ifng in cell culture supernatants and serum. Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0375)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H23-C155

Sensitivity

<5 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0375 is reactive to Ifng in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0375 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0375-CAP Anti-Mouse Ifng Pre-coated 96-well strip microplate 1
EK0375-ST Mouse Ifng Standard 2 vials, 10 ng/tube
EK0375-DA Mouse Ifng Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0490.1420.2530.3750.5951.0151.3551.997

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IFN-gamma, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IFN-gamma in samples: 25650554 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6822476070232686
Standard deviation2.9212.3233.443.0812.7630.18
CV (%)4.3%5.5%4.4%4.4%5.5%4.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IFN-gamma in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 168696665671.582.3%
Sample 222420320824321915.627.1%
Sample 376083973188380360.687.5%
*number of samples for each test n=16.

Gene/Protein Information For Ifng (Source: Uniprot.Org, NCBI)

Uniprot ID

P01580

Gene ID

15978

Gene Name

Ifng

Full Name

Interferon gamma

Weight

17.907kDa

Superfamily

type II (or gamma) interferon family

Alternative Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma Ifng|IFN-g, If, If2f, Ifg|interferon gamma|interferon gamma|IFN-gamma|gamma interferon|interferon 2f

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ifng, check out the Ifng Infographic

Ifng infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ifng: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0375 has been cited in 60 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

In vivo study of immunogenicity and kinetic characteristics of a quantum dot-labelled baculovirus
Species: Mouse

GSH depletion liposome adjuvant for augmenting the photothermal immunotherapy of breast cancer
Species: Mouse

Laser Immunotherapy in Combination with Perdurable PD-1 Blocking for the Treatment of Metastatic Tumors
Species: Mouse

Synergistic Enhancement of Lung Cancer Therapy Through Nanocarrier‐Mediated Sequential Delivery of Superantigen and Tyrosin Kinase Inhibitor
Species: Mouse

Ultrasmall Graphene Oxide Supported Gold Nanoparticles as Adjuvants Improve Humoral and Cellular Immunity in Mice
Species: Mouse

Complete eradication of hepatocellular carcinomas by combined vasostatin gene therapy and B7H3-mediated immunotherapy
Species: Mouse

Qiu-yue Fang,Shu-ping Chen,Jun-qiao Wang,Xiao-jun Huang,Qi-xing Nie,Glyn O. Philips,Steve W. Cui,Ya-jing Li,Shao-ping Nie,Fractions from natural Cordyceps sinensis alleviated intestinal injury in Cyclophosphamide-induced mice,Bioactive Carbohydrates and Dietary Fibre,2021,100271,ISSN 2212-6198,https://doi.org/10.1016/j.bcdf.2021.100271.
Species: Mouse
EK0375 usage in article: APP:ELISA, SAMPLE:SMALL INTESTINE, DILUTION:NA

Tawfiq,R.A.,Attia,Y.M.,Nassar,N.N.,Hammam,O.A.,Elmazar,M.M.,& Abdallah,D.M.(2021).Insights into the Impact of FXR Activation on Hepatic Autophagy in a Non-alcoholic Steatohepatitis Model.Bulletin of Pharmaceutical Sciences.Assiut,44(1),265-274.
Species: Mouse
EK0375 usage in article: APP:ELISA, SAMPLE:LIVER TISSUE, DILUTION:NA

Wan Y,Wu W,Wan Y,Zhang J,Li L,Chen X,Liu S,Yao X.Brivanib alaninate inhibited dengue virus proliferation through VEGFR2/AMPK pathway.Pharmacol Res. 2021 Jun 8:105721.doi:10.1016/j.phrs.2021.105721.Epub ahead of print.PMID:34116207.
Species: Hamster

Ze-Hui Liu,Hui-Ling Xu,Guang-Wei Han,Li-Na Tao,Ying Lu,Su-Ya Zheng,Wei-Huan Fang,Fang He,A self-assembling nanoparticle: Implications for the development of thermostable vaccine candidates,International Journal of Biological Macromolecules,2021,,ISSN 0141-8130,https://doi.org/10.1016/j.ijbiomac.2021.06.024.
Species: Mouse
EK0375 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Xin Zhang,Xiuting Liu,Wei Zhou,Qianming Du,Mengdi Yang,Yang Ding,Rong Hu,Blockade of IDO-kynurenine-AhR axis ameliorated colitis-associated colon cancer via inhibiting immune tolerance,Cellular and Molecular Gastroenterology and Hepatology,2021,,ISSN 2352-345X,https://doi.org/10.1016/j.jcmgh.2021.05.018.
Species: Human,Mouse
EK0375 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA

Feng Z,Peng S,Wu Z,Jiao L,Xu S,Wu Y,Liu Z,Hu Y,Liu J,Wu Y,Wang D.Ramulus mori polysaccharide-loaded PLGA nanoparticles and their anti-inflammatory effects in vivo.Int J Biol Macromol.2021 Jun 1:S0141-8130(21)01184-3.doi:10.1016/j.ijbiomac.2021.05.200.Epub ahead of print.PMID:34087293.
Species: Mouse
EK0375 usage in article: APP:ELISA, SAMPLE:COLON, DILUTION:NA

Zhang Y,Wang R,Shi W,Zhihui Z,Wang X,Li C,Zhang S,Zhang P. Antiviral effect of fufang yinhua jiedu (FFYH) granules against influenza A virus through regulating the inflammatory responses by TLR7/MyD88 signaling pathway.J Ethnopharmacol.2021 Apr 1:114063.doi:10. 1016/j.jep.2021.114063.Epub ahead of print.PMID:33813013.
Species: Mouse,Canine
EK0375 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Attia YM, Tawfiq RA, Gibriel AA, Ali AA, Kassem DH, Hammam OA, Elmazar MM. Activation of FXR modulates SOCS3/Jak2/STAT3 signaling axis in a NASH-dependent hepatocellular carcinoma animal model. Biochem Pharmacol. 2021 Mar 3:114497. doi: 10.1016/j.bcp.2021.114497. Epub ahead of print. PMID: 33675775.
Species: Mouse
EK0375 usage in article: APP:ELISA, SAMPLE:LIVER TISSUE, DILUTION:NA

Qiu F,Lu W,Ye S,Liu H,Zeng Q,Huang H,Liang CL,Chen Y,Zheng F,Zhang Q,Lu CJ,Dai Z. Berberine Promotes Induction of Immunological Tolerance to an Allograft via Downregulating Memory CD8+ T-Cells Through Altering the Gut Microbiota. Front Immunol.2021 Feb 12
Species: Mouse
EK0375 usage in article: APP:ELISA, SAMPLE:CD3 CELL AND T CELL, DILUTION:NA

Xi Y,Ma Y,Xie B,Di A,Xu S,Luo X,Wang C,Dai H,Yan G,Qi Z.Vitamin D3 combined with antibody agents suppresses alloreactive memory T-cell responses to induce heart allograft long-term survival.Transpl Immunol.2021 Feb 13:101374.doi:10.1016/j.trim.2021.101374
Species: Mouse

Zhang Z,Song X,Zhang Z,Li H,Duan Y,Zhang H,Lu H,Luo C, Wang M.The molecular characterization and immune protection of adhesion protein 65 (AP65) of Trichomonas vaginalis.Microb Pathog.2021 Jan 20:104750.doi:10.1016/j.micpath.2021.104750.Epub ahead of prin
Species: Mouse,Rat
EK0375 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

ELGENDY, D.,OTHMAN, A.,EL-GUINDY, D.,SOLIMAN, N.,ELMEHY, D.(2020).SYNERGY BETWEEN BETA-1, 3-GLUCAN AND PRAZIQUANTEL IN THE TREATMENT OF EXPERIMENTAL CHRONIC SCHISTOSOMIASIS MANSONI.Journal of the Egyptian Society of Parasitology,50(3),565-574.doi:10.12816
Species: Mouse
EK0375 usage in article: APP:ELISA, SAMPLE:LIVER TISSUE, DILUTION:NA

Mingyue Shen,Xianxiang Chen,Lixin Huang,Qiang Yu,Yi Chen,Jianhua Xie,Sulfated Mesona chinensis Benth polysaccharide enhance the immunomodulatory activities of cyclophosphamide-treated mice,Journal of Functional Foods,Volume 76,2021,104321,ISSN 1756-4646,h
Species: Mouse
EK0375 usage in article: APP:ELISA, SAMPLE:SPLENIC LYMPHOCYTES, DILUTION: NA

Zhang Z,Jiang S,Tian H,Zeng Y,He K,Lin L,Yu F. Ethyl acetate fraction from Nymphaea hybrida Peck modulates inflammatory responses in LPS-stimulated RAW 264.7 cells and acute inflammation murine models. J Ethnopharmacol. 2020 Dec 15:113698.doi:10.1016/j.je
Species: Mouse, Nymphaea hybrida
EK0375 usage in article: APP:ELISA, SAMPLE: RAW264.7 CELLS, DILUTION:NA

Wu CY, Chuang HY, Wong CH. Influenza virus neuraminidase regulates host CD8+ T-cell response in mice. Commun Biol. 2020 Dec 8;3(1):748. doi: 10.1038/s42003-020-01486-z. PMID: 33293641; PMCID:PMC772285 4.
Species: Human, Mouse, Dog
EK0375 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Sun H, Fei L, Zhu B, Shi M. Quick and improved immune responses to inactivated H9N2 avian influenza vaccine by purified active fraction of Albizia julibrissin saponins. BMC Vet Res. 2020 Nov 7;16(1):427. doi: 10.1186/s12917-020-02648-1. PMID: 33160337; PM
Species: Mouse,Chicken
EK0375 usage in article: APP:ELISA, SAMPLE:SPLENOCYTES, DILUTION:NA

Huang L,Shen M,Wu T,Yu Y,Yu Q,Chen Y,Xie J.Mesona chinensis Benth polysaccharides protect against oxidative stress and immunosuppression in cyclophosphamide-treated mice via MAPKs signal transduction pathways.Int J Biol Macromol.2020 Jun 1;152:766-774. do
Species: Mesona chinensis, Mouse
EK0375 usage in article: APP:ELISA, SAMPLE:SPLENIC LYMPHOCYTES, DILUTION:NA

Antiasthmatic Effects of Sanglong Pingchuan Decoction through Inducing a Balanced Th1/Th2 Immune Response

The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells

A novel derivative of (-) mycousnine produced by the endophytic fungus Mycosphaerella nawae, exhibits high and selective immunosuppressive activity on T %u2026

Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding?Toxoplasma gondii?elongation factor 1-alpha

3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation

Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis

5-BDBD ameliorates an OVA-induced allergic asthma by the reduction of Th2 cytokines production

Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1?) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis

Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection

A deleterious role for Th9/IL-9 in hepatic fibrogenesis

Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica

Stimulation of dendritic cells by DAMPs in ALA-PDT treated SCC tumor cells

Immunostimulative Activity of Low Molecular Weight Chitosans in RAW264.7 Macrophages

3-(3-Pyridylmethylidene)-2-indolinone Reduces the Severity of Colonic Injury in a Murine Model of Experimental Colitis

Virus-Like Particles of Chimeric Recombinant Porcine Circovirus Type 2 as Antigen Vehicle Carrying Foreign Epitopes

DNA vaccination with a gene encoding?Toxoplasma gondii?Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice

Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model

Construction and Immunogenicity of DNA Vaccines Encoding Fusion Protein of Porcine IFN-?1 and GP5 Gene of Porcine Reproductive and Respiratory Syndrome Virus

Hybrid phage displaying SLAQVKYTSASSI induces protection against?Candida albicans?challenge in BALB/c mice

A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and?Staphylococcus aureus

Adenovirus-mediated delivery of interferon-? gene inhibits the growth of nasopharyngeal carcinoma

Molecular Adjuvant Ag85A Enhances Protection against Influenza A Virus in Mice Following DNA Vaccination

Xu B, Zhang P, Li W, Liu R, Tang J, Fan H. Front Microbiol. 2017 Aug 9;8:1524. doi: 10.3389/fmicb.2017.01524. eCollection 2017. hsdS, Belonging to the Type I Restriction-Modification System, Contributes to the Streptococcus suis Serotype 2 Surviva...

Qin S, Gao Z, Liu Y, Liu C, Wang J, Zou LL. Oncol Lett. 2018 Feb;15(2):1630-1638. doi: 10.3892/ol.2017.7477. Epub 2017 Nov 23. Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cel...

Wang S1, Zhang H1, Xi Z1, Huang J1, Nie J1, Zhou B1, Deng Y2, Tao Z2. Exp Ther Med. 2017 Dec;14(6):5275-5282. doi: 10.3892/etm.2017.5208. Epub 2017 Sep 27. Establishment of a mouse model of lipopolysaccharide-induced neutrophilic nasal polyps

Lu C, Liu H, Jin X, Chen Y, Liang CL, Qiu F, Dai Z. Front Pharmacol. 2018 Feb 12;9:88. doi: 10.3389/fphar.2018.00088. eCollection 2018. Herbal Components of a Novel Formula PSORI-CM02 Interdependently Suppress Allograft Rejection and Induce CD8+CD...

Liang, Y., Song, D.Z., Liang, S., Zhang, Z.F., Gao, L.X., & Fan, X.H. (2017). The hemagglutinin-neuramidinase protein of Newcastle disease virus upregulates expression of the TRAIL gene in murine natural killer cells through the activation of Syk ...

Liu, C., Huang, R., Yao, R., & Yang, A. (2017). The Immunotherapeutic Role of Bacterial Lysates in a Mouse Model of Asthma. Lung, 1-7. Advance online publication. doi: 10.1007/s00408-017-0003-8.

He K, Liu P, Xu LX. Cell Death Dis. 2017 Mar 23;8(3):e2703. doi: 10.1038/cddis.2017.125.The cryo-thermal therapy eradicated melanoma in mice by eliciting CD4+ T-cell-mediated antitumor memory immune response.

Yang B, Zhou X, Lan C. Bmc Gastroenterol. 2015 Apr 1;15:43. Doi: 10.1186/S12876-015-0272-8. Changes Of Cytokine Levels In A Mouse Model Of Post-Infectious Irritable Bowel Syndrome.

Jin Y, Zhang Y, Wan C, Wang H, Hou L, Chang J, Fan K, Xie X. Evid Based Complement Alternat Med. 2013;2013:194976. Doi: 10.1155/2013/194976. Epub 2013 Sep 18. Immunomodulatory Activity And Protective Effects Of Polysaccharide From Eupatorium Adeno...

Lu Dh, Guo Xy, Qin Sy, Luo W, Huang Xl, Chen M, Wang Jx, Ma Sj, Yang Xw, Jiang Hx. World J Gastroenterol. 2015 Feb 7;21(5):1531-45. Doi: 10.3748/Wjg.V21.I5.1531. Interleukin-22 Ameliorates Liver Fibrogenesis By Attenuating Hepatic Stellate Cell Ac...

Zheng Ls, Kaneko N, Sawamoto K. Front Cell Neurosci. 2015 Jan 28;9:5. Doi: 10.3389/Fncel.2015.00005. Ecollection 2015. Minocycline Treatment Ameliorates Interferon-Alpha- Induced Neurogenic Defects And Depression-Like Behaviors In Mice.

Zhong G, Cheng X, Long H, He L, Qi W, Xiang T, Zhao Z, Zhu B. J Transl Med. 2013 Mar 21;11:71. Doi: 10.1186/1479-5876-11-71. Dynamically Expressed Microrna-15B Modulates The Activities Of Cd8+ T Lymphocytes In Mice With Lewis Lung Carcinoma.

Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...

Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide.

Meng Fy, Ning Yl, Qi J, He Z, Jie J, Lin Jj, Huang Yj, Li Fs, Li Xh. Int J Mol Sci. 2014 Mar 24;15(3):5140-62. Doi: 10.3390/Ijms15035140. Structure And Antitumor And Immunomodulatory Activities Of A Water-Soluble Polysaccharide From Dimocarpus Lon...

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7 Customer Q&As for Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for IFN-gamma ELISA kit? I used your IFN-gamma ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IFN-gamma even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-10-23

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IFN-gamma ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of IFN-gamma that can be declared/interpreted as positive by the above standard is 5pg/ml.

Boster Scientific Support

Answered: 2020-10-23

Question

Can EK0375 be performed on frozen serum?

Verified customer

Asked: 2020-09-18

Answer

The Mouse IFN Gamma / IFNG / Interferon Gamma ELISA Kit PicoKine™ (EK0375) can be performed on frozen serum. Please run a pilot test with a small portion of the samples first.

Boster Scientific Support

Answered: 2020-09-21

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-04-13

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of IFN-gamma in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-11-24

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IFN-gamma ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-11-24

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification IFN-gamma using the EK0375 Mouse IFN-gamma Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-02-10

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2018-02-10

Question

Q: how to proceed with the analysis of ELISA data? I have performed IFN-gamma level in serum.

Verified Customer

Verified customer

Asked: 2015-03-05

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2015-03-05

Question

Q: if the enzyme conjugated IFN-gamma antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IFN-gamma antigen?

Verified Customer

Verified customer

Asked: 2014-04-15

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2014-04-15

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