Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine®

IFN-gamma ELISA kit for Mouse

Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ifng in cell culture supernatants and serum. Sensitivity: 5pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0375
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine®

View all IFN-gamma ELISA kits

SKU/Catalog Number

EK0375

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ifng in cell culture supernatants and serum. Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0375)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H23-C155

Sensitivity

<5 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0375 is reactive to Ifng in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0375 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IFN-gamma

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Kit Components

Catalog Number Description Quantity
EK0375-CAP Anti-Mouse Ifng Pre-coated 96-well strip microplate 1
EK0375-ST Mouse Ifng Standard 2 vials, 10 ng/tube
EK0375-DA Mouse Ifng Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0490.1420.2530.3750.5951.0151.3551.997

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IFN-gamma, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IFN-gamma in samples: 25650554 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6822476070232686
Standard deviation2.9212.3233.443.0812.7630.18
CV (%)4.3%5.5%4.4%4.4%5.5%4.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IFN-gamma in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 168696665671.582.3%
Sample 222420320824321915.627.1%
Sample 376083973188380360.687.5%
*number of samples for each test n=16.

Gene/Protein Information For Ifng (Source: Uniprot.Org, NCBI)

Uniprot ID

P01580

Gene ID

15978

Gene Name

Ifng

Full Name

Interferon gamma

Weight

17.907kDa

Superfamily

type II (or gamma) interferon family

Alternative Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma Ifng|IFN-g, If, If2f, Ifg|interferon gamma|interferon gamma|IFN-gamma|gamma interferon|interferon 2f

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ifng, check out the Ifng Infographic

Ifng infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ifng: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0375 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Dihydroartemisinin ameliorates psoriatic skin inflammation and its relapse by diminishing CD8+ T-cell memory in wild-type and humanized mice

A Synthetic Receptor as a Specific Antidote for Paraquat Poisoning

Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice

Melanoma B16-F1 cells coated with fusion protein of mouse calreticulin and virus G-protein coupled receptor induced the antitumor immune response in Balb/C mice

Intranasal immunization with recombinant Lactococci carrying human papillomavirus E7 protein and mouse interleukin‑12 DNA induces E7‑specific antitumor effects in C57BL/6 mice

Alteration of T helper cell subsets in the optic nerve of experimental autoimmune encephalomyelitis

Calreticulin is an effective immunologic adjuvant to tumor‑associated antigens

Apoptotic Melanoma B16-F1 Cells Induced by Lidamycin Could Initiate the Antitumor Immune Response in BABL/c Mice

Identification of Toxoplasma Gondii Tyrosine Hydroxylase (TH) Activity and Molecular Immunoprotection against Toxoplasmosis

Virus-Like Particles of Chimeric Recombinant Porcine Circovirus Type 2 as Antigen Vehicle Carrying Foreign Epitopes

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7 Customer Q&As for Mouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine®

Question

Q: What is the optimal O.D. value for IFN-gamma ELISA kit? I used your IFN-gamma ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IFN-gamma even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-10-23

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IFN-gamma ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of IFN-gamma that can be declared/interpreted as positive by the above standard is 5pg/ml.

Boster Scientific Support

Answered: 2020-10-23

Question

Can EK0375 be performed on frozen serum?

Verified customer

Asked: 2020-09-18

Answer

The Mouse IFN Gamma / IFNG / Interferon Gamma ELISA Kit PicoKine™ (EK0375) can be performed on frozen serum. Please run a pilot test with a small portion of the samples first.

Boster Scientific Support

Answered: 2020-09-21

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-04-13

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of IFN-gamma in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-11-24

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IFN-gamma ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-11-24

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification IFN-gamma using the EK0375 Mouse IFN-gamma Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-02-10

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2018-02-10

Question

Q: how to proceed with the analysis of ELISA data? I have performed IFN-gamma level in serum.

Verified Customer

Verified customer

Asked: 2015-03-05

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2015-03-05

Question

Q: if the enzyme conjugated IFN-gamma antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IFN-gamma antigen?

Verified Customer

Verified customer

Asked: 2014-04-15

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2014-04-15

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