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- Table of Contents
IRF3 antibody (A00165-7) performs well in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of IRF3 using anti-IRF3 antibody (A00165-7).
IRF3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRF3 Antibody (A00165-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
IRF3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRF3 Antibody (A00165-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-IRF3 Antibody Picoband®
IRF3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
IRF3 antibody was positively detected by WB in:
human Hela whole cell, human Jurkat whole cell, human Raji whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
IRF3 antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human bladder urothelial carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
ICC
IF
IP
ELISA
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Recommend
Mouse
Recommend
Recommend
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-IRF3 Antibody Picoband®
View all IRF3 Antibodies
View all IRF3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IRF3 recombinant protein (Position: T3-L450).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50-55 kDa
Calculated Molecular Weight
47.2 kDa
Background of IRF3
IRF3(interferon regulatory factor 3)is a member of the interferon regulatory transcription factor(IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex withCREBBP. IRF3 plays an important role in theinnate immune system's response toviral infection. AggregatedMAVShave been found to activate IRF3 dimerization. AlthoughIRF3increased transcriptional activity from an ISRE-containing promoter, expression ofIRF3as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase(CAT) reporter gene containing repeats of the Gal4-binding sites.Translocation ofIRF3was accompanied by an increase in serine and threonine phosphorylation.The transcriptional activators CREBBP and EP300 coimmunoprecipitated withIRF3only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.
Cite This Product
Anti-IRF3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00165-7)
Protocols
Western Blot protocol for IRF3 antibody
Immunohistochemistry (IHC) protocol for IRF3 antibody
Publications
Application:
Species:
Electroacupuncture protects gastric Cajal cells by reducing macrophage pyroptosis in diabetic gastroparesis
Published: 2026-02-15
Journal: World Journal of Diabetes
Application: WB
Impact Factor: 4.60
Species: Rat
PMID: 41696103
In Situ Programming of the Tumor Microenvironment to Alleviate Immunosuppression for Pancreatic Cancer Immunotherapy
Published: 2025-06-23
Journal: Advanced Science
Application: WB
Impact Factor: 14.10
Species: Mouse
PMID: 40548883
Evolutionarily conserved Otub1 suppresses antiviral immune response by promoting Irf3 proteasomal degradation in miiuy croaker, Miichthys miiuy
Published: 2024-06-22
Journal: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Application: WB
Impact Factor: 2.70
Species: Fish
PMID: 38914152
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Product Promise
We promise Anti-IRF3 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00165-7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026