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Product Info Summary
| SKU: | A05068 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-JIP-1 MAPK8IP1 Antibody
SKU/Catalog Number
A05068
Size
100μl
Form
Liquid
Description
Boster Bio Anti-JIP-1 MAPK8IP1 Antibody catalog # A05068. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JIP-1 MAPK8IP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05068)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human JIP1. AA range:69-118
Reactive Species
A05068 is reactive to MAPK8IP1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
77.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05068 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of COLO205 cells using JIP-1 Polyclonal Antibody
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Western blot analysis of lysates from COLO205 cells, treated with Serum 20% 15', using JIP1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using JIP1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of NIH/3T3 cells, using JIP1 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-JIP-1 MAPK8IP1 Antibody (A05068)
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Customer Q&As
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5 Customer Q&As for Anti-JIP-1 MAPK8IP1 Antibody
Question
I see that the anti-JIP-1 antibody A05068 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-30
Question
I was wanting to use to test anti-JIP-1 antibody A05068 on rat insulinoma for research purposes, then I may be interested in using anti-JIP-1 antibody A05068 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
The products we sell, including anti-JIP-1 antibody A05068, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-26
Question
Is this A05068 anti-JIP-1 antibody reactive to the isotypes of MAPK8IP1?
F. Williams
Verified customer
Asked: 2018-08-07
Answer
The immunogen of A05068 anti-JIP-1 antibody is Synthesized peptide derived from human JIP-1 around the non-phosphorylation site of T103. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-07
Question
We are currently using anti-JIP-1 antibody A05068 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
The anti-JIP-1 antibody (A05068) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-24
Question
Does anti-JIP-1 antibody A05068 work on horse WB with insulinoma?
T. Parker
Verified customer
Asked: 2015-10-01
Answer
Our lab technicians have not validated anti-JIP-1 antibody A05068 on horse. You can run a BLAST between horse and the immunogen sequence of anti-JIP-1 antibody A05068 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse insulinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-10-01
