Anti-MAP3K4/Mekk4 Antibody

Boster Bio Anti-MAP3K4/Mekk4 Antibody catalog # A04842. Tested in IHC applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A04842
Size: 100ul
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC

Product Name

Anti-MAP3K4/Mekk4 Antibody

See all MEKK4 products

SKU/Catalog Number

A04842

Size

100ul

Form

Liquid

Description

Boster Bio Anti-MAP3K4/Mekk4 Antibody catalog # A04842. Tested in IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MAP3K4/Mekk4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04842)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

A peptide derived from human MAP3K4. Immunogen sequence location: 1281-1330

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04842 is reactive to MAP3K4 in Human, Mouse

Applications

A04842 is guaranteed for IHC Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

User need to optimize the dilution ratio for this antibody

Validation Images & Assay Conditions

Gene/Protein Information For MAP3K4 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9Y6R4

Gene ID

4216

Gene Name

MAP3K4

Full Name

Mitogen-activated protein kinase kinase kinase 4

Weight

181.685kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11.25; FLJ42439; MAPKKK4yeast, homolog of; MEKK 4; mitogen-activated protein kinase kinase kinase 4 MAP3K4 MAPKKK4, MEKK 4, MEKK4, MTK1, PRO0412 mitogen-activated protein kinase kinase kinase 4 mitogen-activated protein kinase kinase kinase 4|MAP three kinase 1|MAP/ERK kinase kinase 4|MAPK/ERK kinase kinase 4|MEK kinase 4|SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of|dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4)|predicted protein of HQ0412

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MAP3K4, check out the MAP3K4 Infographic

MAP3K4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MAP3K4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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13 Customer Q&As for Anti-MAP3K4/Mekk4 Antibody

Question

We are currently using anti-MAP3K4/Mekk4 antibody A04842 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

The anti-MAP3K4/Mekk4 antibody (A04842) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-18

Question

Is this A04842 anti-MAP3K4/Mekk4 antibody reactive to the isotypes of MAP3K4?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

The immunogen of A04842 anti-MAP3K4/Mekk4 antibody is A peptide derived from human MAP3K4. Immunogen sequence location: 1281-1330. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-03

Question

Will anti-MAP3K4/Mekk4 antibody A04842 work for IHC with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

According to the expression profile of leukemic t-cell, MAP3K4 is highly expressed in leukemic t-cell. So, it is likely that anti-MAP3K4/Mekk4 antibody A04842 will work for IHC with leukemic t-cell.

Boster Scientific Support

Answered: 2019-10-04

Question

Does anti-MAP3K4/Mekk4 antibody A04842 work on goat IHC with cerebellum?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

Our lab technicians have not validated anti-MAP3K4/Mekk4 antibody A04842 on goat. You can run a BLAST between goat and the immunogen sequence of anti-MAP3K4/Mekk4 antibody A04842 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cerebellum in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-03

Question

My question regarding product A04842, anti-MAP3K4/Mekk4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04842 anti-MAP3K4/Mekk4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-29

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-MAP3K4/Mekk4 antibody A04842. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-12

Question

Would A04842 anti-MAP3K4/Mekk4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

It shows on the product datasheet, A04842 anti-MAP3K4/Mekk4 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-22

Question

Is a blocking peptide available for product anti-MAP3K4/Mekk4 antibody (A04842)?

C. Johnson

Verified customer

Asked: 2018-01-29

Answer

We do provide the blocking peptide for product anti-MAP3K4/Mekk4 antibody (A04842). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-01-29

Question

See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-MAP3K4/Mekk4 antibody A04842. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-08-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-21

Question

I see that the anti-MAP3K4/Mekk4 antibody A04842 works with IHC, what is the protocol used to produce the result images on the product page?

G. Patel

Verified customer

Asked: 2017-02-15

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-02-15

Question

I was wanting to use your anti-MAP3K4/Mekk4 antibody for IHC for mouse leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukemic t-cell identification?

N. Zhao

Verified customer

Asked: 2016-07-05

Answer

As indicated on the product datasheet, A04842 anti-MAP3K4/Mekk4 antibody has been validated for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-07-05

Question

Is there a BSA free version of anti-MAP3K4/Mekk4 antibody A04842 available?

G. Johnson

Verified customer

Asked: 2015-04-29

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MAP3K4/Mekk4 antibody A04842 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-04-29

Question

My question regards to test anti-MAP3K4/Mekk4 antibody A04842 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-MAP3K4/Mekk4 antibody A04842 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

B. Parker

Verified customer

Asked: 2013-02-22

Answer

The products we sell, including anti-MAP3K4/Mekk4 antibody A04842, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-02-22

Size

Total: $323

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A04842
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$323.00

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