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Product Info Summary
| SKU: | A01309 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-JMJD3 KDM6B Antibody
SKU/Catalog Number
A01309
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-JMJD3 KDM6B Antibody (Catalog # A01309). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
JMJD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-JMJD3 KDM6B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01309)
Host
Rabbit
Contents
JMJD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-JMJD3 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human JMJD3. The immunogen is located within amino acids 1370-1420 of JMJD3.
Cross-reactivity
This antibody is specific for JMJD3 and will not recognize other JMJD proteins.
Reactive Species
A01309 is reactive to KDM6B in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
176.6 kDa
Background of KDM6B
The Jumonji domain-containing protein 3 (JMJD3) functions as a trimethylation-specific demethylase, converting the trimethylated histone H3 Lys27 residue to the dimethylated form, and is thought to also function as a transcriptional repressor. JMJD3 plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. JMJD3 can also interact with and demethylate p53, resulting in its stabilization and localization to the nucleus in mouse embryo fibroblasts during neural stem cell differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01309 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.25-4 μg/mL; ICC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Human, Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: JMJD3, A01309, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: JMJD3 A01309 (1 μg/mL), JMJD3 14-618, (2 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in HeLa Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: JMJD3, A01309 (0.25 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in K562 Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: : JMJD3, A01309 (0.5 μg/mL) , 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Lane 1: in the absence and Lane 2: in the presence of blocking peptide
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Western Blot Validation in Mouse A-20 Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: : JMJD3, A01309 (0.5 μg/mL) , 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Lane 1: in the absence and Lane 2: in the presence of blocking peptide
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Western Blot Validation in HepG2 Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: : JMJD3, A01309, 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Lane 1: 0.25 μg/mL
Lane 2: 0.5 μg/mL
Lane 3: 0.25 μg/mL with blocking peptide
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Immunocytochemistry Validation of JMJD3 in K562 Cells
Immunocytochemical analysis of K562 cells using anti-JMJD3 antibody (A01309) at 2.5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of JMJD3 in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling JMJD3 with A01309 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Specific Publications For Anti-JMJD3 KDM6B Antibody (A01309)
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