Click image to see more details
Product Info Summary
| SKU: | A01036-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-KCNH1 Antibody
SKU/Catalog Number
A01036-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-KCNH1 Antibody catalog # A01036-1. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KCNH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01036-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human KCNH1. AA range:720-769
Reactive Species
A01036-1 is reactive to KCNH1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
111.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01036-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western blot analysis of KCNH1 Antibody. The lane on the right is blocked with the KCNH1 peptide.
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Western blot analysis of the lysates from COLO205 cells using KCNH1 antibody.
Specific Publications For Anti-KCNH1 Antibody (A01036-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-KCNH1 Antibody
Question
Will anti-KCNH1 antibody A01036-1 work for WB with myoblast?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
According to the expression profile of myoblast, KCNH1 is highly expressed in myoblast. So, it is likely that anti-KCNH1 antibody A01036-1 will work for WB with myoblast.
Boster Scientific Support
Answered: 2020-03-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for myoblast using anti-KCNH1 antibody A01036-1. Let me know if you need anything else.
P. Zhang
Verified customer
Asked: 2020-03-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-18
Question
I was wanting to use your anti-KCNH1 antibody for WB for rat myoblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat myoblast identification?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
It shows on the product datasheet, A01036-1 anti-KCNH1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat myoblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-05
Question
Can you help my question with product A01036-1, anti-KCNH1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01036-1 anti-KCNH1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-08
Question
Does anti-KCNH1 antibody A01036-1 work on pig WB with myoblast?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
Our lab technicians have not validated anti-KCNH1 antibody A01036-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-KCNH1 antibody A01036-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig myoblast in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-27
Question
Is there a BSA free version of anti-KCNH1 antibody A01036-1 available?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-KCNH1 antibody A01036-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-19
Question
I see that the anti-KCNH1 antibody A01036-1 works with WB, what is the protocol used to produce the result images on the product page?
B. Kulkarni
Verified customer
Asked: 2018-08-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-08-17
