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Product Info Summary
| SKU: | M00514 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-KEAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4]
SKU/Catalog Number
M00514
Size
100 µl
Description
Boster Bio KEAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4). Catalog# M00514. Tested in Flow Cytometry, IF, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-KEAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00514)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1B4
Isotype
IgG1
Immunogen
Full length human recombinnt protein of human KEAP1 (NP_987096) produced in HEK293T cell.
Reactive Species
M00514 is reactive to KEAP1 in Human, Mouse, Rat
Calculated molecular weight
69.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00514 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000, FLOW 1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts (50ug per lane) from 5 cell lines lysates by using anti-TMEM173 monoclonal antibody(M00514, 1:500)
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Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid, lane 1) , human KEAP1 plasmid, lane 2), mouse KEAP1 plasmid transcript variant 2, lane 3), mouse KEAP1 plasmid transcript variant 4, lane 4), rat KEAP1 plasmid, lane 5) using antiKEAP1 antibody M00514 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody, 1:1000)
Specific Publications For Anti-KEAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4] (M00514)
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