Anti-KU80/XRCC5 Antibody (Monoclonal, 31X78)

Product Name

Anti-KU80/XRCC5 Antibody (Monoclonal, 31X78)

View all XRCC5 Antibodies

SKU/Catalog Number

M01275-3

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-KU80/XRCC5 Antibody (Monoclonal, 31X78) catalog # M01275-3. Tested in WB, IHC, IF, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Monkey.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KU80/XRCC5 Antibody (Monoclonal, 31X78) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01275-3)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

31X78

Immunogen

Synthetic peptide within Human Ku80 aa 695-730.

Reactive Species

M01275-3 is reactive to XRCC5 in Human, Monkey

Calculated molecular weight

82.7 kDa

Background of XRCC5

XRCC5 (X-ray Repair,Complementing Defective ,In Chinese Hamster, 5), also known as Ku80 or Ku86, is a protein that in humans, is encoded by the XRCC5 gene. The XRCC5 gene encodes the 80-kD subunit of the Ku autoantigen, a heterodimer which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end joining (NHEJ) pathway. The XRCC5 gene is mapped to 2q35. Human colon cancer cells heterozygous for Ku86 are haploinsufficient with an increase in polyploid cells, a reduction in cell proliferation, elevated p53 levels, and a slight hypersensitivity to ionizing radiation. Functional inactivation of the second Ku86 allele results in cells with a drastically reduced doubling time. The Ku86 locus is essential in human somatic tissue culture cells by experiments demonstration. A rare microsatellite polymorphism in XRCC5 is associated with cancer in patients of varying radiosensitivity.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01275-3 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunofluorescence, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200
ImmunoPrecipitation, 1:50
Flow Cytometry (Fixed), 1:50-200

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information XRCC5

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