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Product Info Summary
| SKU: | A09889 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Kv3.4 KCNC4 Antibody
SKU/Catalog Number
A09889
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Kv3.4 KCNC4 Antibody catalog # A09889. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kv3.4 KCNC4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09889)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human KCNC4. AA range:1-50
Reactive Species
A09889 is reactive to KCNC4 in Human, Monkey, Mouse
Calculated molecular weight
69.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09889 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of SH-SY5Y cells using Kv3.4 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from COS7 cells treated with Anisomycin 25ug/ml 30', using Kv3.4/KCNC4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using Kv3.4/KCNC4 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HeLa cells, using Kv3.4/KCNC4 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Kv3.4 KCNC4 Antibody (A09889)
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Customer Q&As
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7 Customer Q&As for Anti-Kv3.4 KCNC4 Antibody
Question
Will A09889 anti-Kv3.4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
You can see on the product datasheet, A09889 anti-Kv3.4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-28
Question
I was wanting to use your anti-Kv3.4 antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
As indicated on the product datasheet, A09889 anti-Kv3.4 antibody has been validated for WB on human, monkey, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-20
Question
We need to test anti-Kv3.4 antibody A09889 on human brain for research purposes, then I may be interested in using anti-Kv3.4 antibody A09889 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
The products we sell, including anti-Kv3.4 antibody A09889, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-18
Question
We are currently using anti-Kv3.4 antibody A09889 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The anti-Kv3.4 antibody (A09889) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-11
Question
I have a question about product A09889, anti-Kv3.4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Li
Verified customer
Asked: 2018-06-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09889 anti-Kv3.4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-13
Question
Does anti-Kv3.4 antibody A09889 work on bovine WB with brain?
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
Our lab technicians have not validated anti-Kv3.4 antibody A09889 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Kv3.4 antibody A09889 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-13
Question
I see that the anti-Kv3.4 antibody A09889 works with WB, what is the protocol used to produce the result images on the product page?
R. Zhao
Verified customer
Asked: 2014-04-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-04-25
