|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Laminin Gamma 1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Laminin subunit gamma-1(LAMC1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Laminin Gamma 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1581)|
|Replacement Item||This antibody may replace the following items: sc-13144|sc-133241|sc-135967|sc-17751|sc-20777|sc-23412|sc-23414|sc-30974|sc-30975|sc-30976|sc-31094|sc-33708|sc-47766|sc-5584|sc-59846|sc-59980|sc-6019|sc-65578|sc-65643|sc-7650 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Laminin gamma 1(1566-1579aa KKQEAAIMDYNRDI), different from the related mouse sequence by one amino acid and rat sequence by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Laminin subunit gamma-1|
|Molecular Weight||177603 MW|
|Protein Function||Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.|
|Tissue Specificity||Found in the basement membranes (major component).|
|Sequence Similarities||Contains 11 laminin EGF-like domains.|
|Subcellular Localization||Secreted, extracellular space, extracellular matrix, basement membrane.|
|Alternative Names||Laminin subunit gamma-1;Laminin B2 chain;Laminin-1 subunit gamma;Laminin-10 subunit gamma;Laminin-11 subunit gamma;Laminin-2 subunit gamma;Laminin-3 subunit gamma;Laminin-4 subunit gamma;Laminin-6 subunit gamma;Laminin-7 subunit gamma;Laminin-8 subunit gamma;Laminin-9 subunit gamma;S-laminin subunit gamma;S-LAM gamma;LAMC1;LAMB2;|
|Research Areas|||cardiovascular|angiogenesis|adhesion / ecm|extracellular matrix| signal transduction|cytoskeleton / ecm|basal lamina||
Background for Laminin subunit gamma-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Laminin Gamma 1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Laminin Gamma 1 Antibody Images
Click the images to enlarge.
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: SKOV1 Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,