Anti-12 Lipoxygenase/ALOX12 UBB Antibody

UBB antibody

Boster Bio Anti-12 Lipoxygenase/ALOX12 UBB Antibody catalog # PA1485-1. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA1485-1
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-12 Lipoxygenase/ALOX12 UBB Antibody

View all UBB Antibodies

SKU/Catalog Number

PA1485-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-12 Lipoxygenase/ALOX12 UBB Antibody catalog # PA1485-1. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-12 Lipoxygenase/ALOX12 UBB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1485-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX12, different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1485-1 is reactive to UBB in Human

Applications

PA1485-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

25.762kDa

Background of UBB

ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNAArachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12 (S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For UBB (Source: Uniprot.org, NCBI)

Gene Name

UBB

Full Name

Polyubiquitin-B

Weight

25.762kDa

Superfamily

ubiquitin family

Alternative Names

RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin UBB HEL-S-50 ubiquitin B polyubiquitin-B|epididymis secretory protein Li 50|polyubiquitin B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on UBB, check out the UBB Infographic

UBB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for UBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-12 Lipoxygenase/ALOX12 UBB Antibody

Question

We are currently using anti-12 Lipoxygenase/ALOX12 antibody PA1485-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?

J. Edwards

Verified customer

Asked: 2016-07-12

Answer

The anti-12 Lipoxygenase/ALOX12 antibody (PA1485-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-07-12

Size

Conjugation

Total: $370

SKU:PA1485-1

In stock, 3+ left.

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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