Product Info Summary
SKU: | PA1485-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-12 Lipoxygenase/ALOX12 UBB Antibody
SKU/Catalog Number
PA1485-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-12 Lipoxygenase/ALOX12 UBB Antibody catalog # PA1485-1. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-12 Lipoxygenase/ALOX12 UBB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1485-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX12, different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1485-1 is reactive to UBB in Human
Applications
PA1485-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
25.762kDa
Background of UBB
ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNAArachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12 (S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-12 Lipoxygenase antibody, PA1485-1, Western blotting
All lanes: Anti ANOX12 (PA1485-1) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 75KD
Observed bind size: 75KD
Protein Target Info & Infographic
Gene/Protein Information For UBB (Source: Uniprot.org, NCBI)
Gene Name
UBB
Full Name
Polyubiquitin-B
Weight
25.762kDa
Superfamily
ubiquitin family
Alternative Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin UBB HEL-S-50 ubiquitin B polyubiquitin-B|epididymis secretory protein Li 50|polyubiquitin B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBB, check out the UBB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-12 Lipoxygenase/ALOX12 UBB Antibody (PA1485-1)
Hello CJ!
No publications found for PA1485-1
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-12 Lipoxygenase/ALOX12 UBB Antibody
Question
We are currently using anti-12 Lipoxygenase/ALOX12 antibody PA1485-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
J. Edwards
Verified customer
Asked: 2016-07-12
Answer
The anti-12 Lipoxygenase/ALOX12 antibody (PA1485-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-12