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Product Info Summary
| SKU: | M01654 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-LAT Purified Monoclonal Antibody
SKU/Catalog Number
M01654
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LAT Purified Monoclonal Antibody (Catalog# M01654). Tested in WB, IP, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-LAT Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01654)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
LAT-01
Isotype
Mouse IgG1
Immunogen
Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT. The antibody LAT-01 reacts with an intracellular epitope of LAT, a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
Reactive Species
M01654 is reactive to LAT in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
27.9 kDa
Background of LAT
LAT (linker for activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01654 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized.
Flow cytometry: 1-4 µg/ml, intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.
Western blotting: 1-2 μg/ml.
Validation Images & Assay Conditions
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Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-LAT (LAT-01) purified antibody (concentration in sample 1 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).
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Separation of human CD3 positive LAT positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-LAT (LAT-01) purified antibody (concentration in sample 1 µg/ml, GAM APC).
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Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-LAT (LAT-01) purified antibody (concentration in sample 1 µg/ml, GAM APC).
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Western blotting analysis of human LAT using mouse monoclonal antibody LAT-01 in lysates of Jurkat cells (positive) and Raji cells (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-LAT monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for LAT at approximately 38 kDa.
Specific Publications For Anti-LAT Purified Monoclonal Antibody (M01654)
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