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- Table of Contents
LDHA antibody (PB10075) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Flow Cytometry analysis of A549 cells using anti-LDHA antibody (PB10075).
Overlay histogram showing A549 cells stained with PB10075 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LDHA Antibody (PB10075,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A549 cells stained with PB10075 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LDHA Antibody (PB10075,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-LDHA Antibody Picoband®
LDHA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
LDHA antibody was positively detected by WB in:
human 293T whole cell, human MCF-7 whole cell, human A375 whole cell, human HepG2 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue, human MSTO-211H- WT whole cell, human MSTO-211H-LDHA KO whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LDHA antibody was positively detected by IHC in:
mouse intestine tissue, rat intestine tissue, human mammary cancer tissue, mouse skeletal tissue, rat muscle skeletal tissue, human stomach cancer tissue, human thyroid cancer tissue, human pancreas cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
LDHA antibody was positively detected by ICC/IF in:
U20S cell
LDHA antibody was positively detected by IF in:
mouse muscle skeletal tissue, rat muscle skeletal tissue, human liver cancer tissue, human stomach cancer tissue, human pancreas cancer tissue
LDHA antibody was positively detected by FC in:
A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-LDHA Antibody Picoband®
View all LDHA Antibodies
View all LDHA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human LDHA recombinant protein (Position: A2-R106). Human LDHA shares 94.3% amino acid (aa) sequence identity with both mouse and rat LDHA.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
37 kDa
Calculated Molecular Weight
36.7 kDa
Background of LDHA
Lactate dehydrogenase A, also known as LDHA, is an enzyme which in humans is encoded by the LDHA gene. The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
Cite This Product
Anti-LDHA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10075)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Mouse
94%
High Homology
Rat
94%
High Homology
Rabbit
94%
High Homology
Sheep
94%
High Homology
Cow
93%
High Homology
Dog
86%
Moderate Homology
Zebrafish
73%
Moderate Homology
Drosophila
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LDHA antibody
Immunohistochemistry (IHC) protocol for LDHA antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for LDHA antibody
Immunofluorescence (IF) protocol for LDHA antibody
Flow Cytometry (FC) protocol for LDHA antibody
Publications
Application:
Species:
Fluoride induced chondrocyte extracellular matrix degradation by inhibition of glycolysis by the PHD2/HIF1α signaling pathway
Published: 2026-08-21
Journal: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Application: WB
Impact Factor: 4.60
Species: Human
PMID: 42628705
Effect and Mechanism of Shenqi Lixin Formula on Angiogenesis and Glucose Metabolism in Rats With Heart Failure After Myocardial Infarction
Published: 2026-01-14
Journal: CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
Application: WB
Impact Factor: 2.50
Species: Rat
PMID: 41533967
Skullcapflavone II Suppresses Colorectal Cancer by Inhibiting the HIF-1α-Glycolysis Pathway
Published: 2025-10-21
Journal: Chemical Biology & Drug Design
Application: WB
Impact Factor: 3.30
Species: Mouse,Human
PMID: 41121650
Impact of SLC16A8 on tumor microenvironment and angiogenesis in colorectal cancer: New therapeutic target insights
Published: 2025-04-15
Journal: World Journal of Gastrointestinal Oncology
Application: WB
Impact Factor: 2.50
Species: Human
PMID: 40235880
Lactate dehydrogenase A is a diagnostic biomarker associated with immune infiltration, m6A modification and ferroptosis in endometrial cancer
Published: 2024-11-08
Journal: Frontiers in Oncology
Application: WB
Impact Factor: 3.50
Species: Human
PMID: 39582531
Characterization of genetic and molecular tools for studying the endogenous expression of Lactate dehydrogenase in Drosophila melanogaster
Published: 2024-01-03
Journal: PLoS One
Application: IF
Impact Factor: 2.90
Species: Drosophila
PMID: 38170735
Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis
Published: 2022-10-13
Journal: iScience
Application: WB
Impact Factor: 6.11
Species: Pig
PMID: 36339254
Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway
Published: 2021-01-01
Journal: International Journal of Medical Sciences
Application: WB
Impact Factor: 3.74
Species: Human
PMID: 33456361
CircPOSTN/miR-361-5p/TPX2 axis regulates cell growth, apoptosis and aerobic glycolysis in glioma cells
Published: 2020-08-06
Journal: Cancer Cell International
Application: WB
Impact Factor: 4.18
Species: Human
PMID: 32774168
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Product Promise
We promise Anti-LDHA Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
PB10075
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026