Product Info Summary
SKU: | RP1075 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-liver Arginase/ARG1 Antibody
View all Arginase 1/ARG1/liver Arginase Antibodies
SKU/Catalog Number
RP1075
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-liver Arginase/ARG1 Antibody catalog # RP1075. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-liver Arginase/ARG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1075)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human liver Arginase, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1075 is reactive to ARG1 in Human, Rat
Applications
RP1075 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
35 kDa
Calculated molecular weight
34.735kDa
Background of Arginase 1/ARG1/liver Arginase
ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liver ARG1 cDNA clone to probe a human liver cDNA library, Haraguchi et al. (1987) isolated and characterized a cDNA corresponding to the ARG1 gene. The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-Liver Arginase antibody, RP1075, Western blotting
All lanes: Anti Liver Arginase (RP1075) at 0.5ug/ml
WB: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 35KD
Observed bind size: 35KD
Protein Target Info & Infographic
Gene/Protein Information For ARG1 (Source: Uniprot.org, NCBI)
Gene Name
ARG1
Full Name
Arginase-1
Weight
34.735kDa
Superfamily
arginase family
Alternative Names
AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase ARG1 arginase 1 arginase-1|arginase, liver|liver-type arginase|type I arginase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARG1, check out the ARG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-liver Arginase/ARG1 Antibody (RP1075)
Hello CJ!
RP1075 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Panax notoginseng Saponins Regulate Macrophage Polarization under Hyperglycemic Condition via NF-%u03BAB Signaling Pathway
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-liver Arginase/ARG1 Antibody
Question
We were satisfied with the WB result of your anti-liver Arginase/ARG1 antibody. However we have observed positive staining in liver cytoplasm. using this antibody. Is that expected? Could you tell me where is ARG1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
According to literature, liver does express ARG1. Generally ARG1 expresses in cytoplasm. Regarding which tissues have ARG1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3174433
Erythroblast, Pubmed ID: 14574404
Liver, Pubmed ID: 2241902, 3540966, 24275569
Liver, and Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-18
Question
We have seen staining in human liver skeletal muscle. Any tips? Is anti-liver Arginase/ARG1 antibody supposed to stain liver skeletal muscle positively?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
From literature liver skeletal muscle does express ARG1. From Uniprot.org, ARG1 is expressed in liver, blood, erythroblast, liver skeletal muscle, among other tissues. Regarding which tissues have ARG1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3174433
Erythroblast, Pubmed ID: 14574404
Liver, Pubmed ID: 2241902, 3540966, 24275569
Liver, and Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-29
Question
We are currently using anti-liver Arginase/ARG1 antibody RP1075 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
The anti-liver Arginase/ARG1 antibody (RP1075) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-15
Question
We are interested in using your anti-liver Arginase/ARG1 antibody for maternal process involved in female pregnancy studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
J. Dhar
Verified customer
Asked: 2015-12-28
Answer
I appreciate your inquiry. This RP1075 anti-liver Arginase/ARG1 antibody is validated on liver tissue, rat liver tissue, tissue lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-12-28