Anti-liver Arginase/ARG1 Antibody

Arginase 1/ARG1/liver Arginase antibody

Boster Bio Anti-liver Arginase/ARG1 Antibody catalog # RP1075. Tested in WB applications. This antibody reacts with Human, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: RP1075
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-liver Arginase/ARG1 Antibody

View all Arginase 1/ARG1/liver Arginase Antibodies

SKU/Catalog Number

RP1075

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-liver Arginase/ARG1 Antibody catalog # RP1075. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-liver Arginase/ARG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1075)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human liver Arginase, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1075 is reactive to ARG1 in Human, Rat

Applications

RP1075 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

34.735kDa

Background of Arginase 1/ARG1/liver Arginase

ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liver ARG1 cDNA clone to probe a human liver cDNA library, Haraguchi et al. (1987) isolated and characterized a cDNA corresponding to the ARG1 gene. The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Human

Validation Images & Assay Conditions

Gene/Protein Information For ARG1 (Source: Uniprot.org, NCBI)

Gene Name

ARG1

Full Name

Arginase-1

Weight

34.735kDa

Superfamily

arginase family

Alternative Names

AI; ARG1; Arginase 1; arginase, liver; Arginase-1; EC 3.5.3.1; Liver Arginase; Liver-type arginase; PGIF; Type I Arginase ARG1 arginase 1 arginase-1|arginase, liver|liver-type arginase|type I arginase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ARG1, check out the ARG1 Infographic

ARG1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ARG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

RP1075 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Panax notoginseng Saponins Regulate Macrophage Polarization under Hyperglycemic Condition via NF-%u03BAB Signaling Pathway

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4 Customer Q&As for Anti-liver Arginase/ARG1 Antibody

Question

We were satisfied with the WB result of your anti-liver Arginase/ARG1 antibody. However we have observed positive staining in liver cytoplasm. using this antibody. Is that expected? Could you tell me where is ARG1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

According to literature, liver does express ARG1. Generally ARG1 expresses in cytoplasm. Regarding which tissues have ARG1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3174433
Erythroblast, Pubmed ID: 14574404
Liver, Pubmed ID: 2241902, 3540966, 24275569
Liver, and Skeletal muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-18

Question

We have seen staining in human liver skeletal muscle. Any tips? Is anti-liver Arginase/ARG1 antibody supposed to stain liver skeletal muscle positively?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

From literature liver skeletal muscle does express ARG1. From Uniprot.org, ARG1 is expressed in liver, blood, erythroblast, liver skeletal muscle, among other tissues. Regarding which tissues have ARG1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3174433
Erythroblast, Pubmed ID: 14574404
Liver, Pubmed ID: 2241902, 3540966, 24275569
Liver, and Skeletal muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-10-29

Question

We are currently using anti-liver Arginase/ARG1 antibody RP1075 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-02-15

Answer

The anti-liver Arginase/ARG1 antibody (RP1075) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-02-15

Question

We are interested in using your anti-liver Arginase/ARG1 antibody for maternal process involved in female pregnancy studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

J. Dhar

Verified customer

Asked: 2015-12-28

Answer

I appreciate your inquiry. This RP1075 anti-liver Arginase/ARG1 antibody is validated on liver tissue, rat liver tissue, tissue lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-12-28

Order DetailsPrice
RP1075

100μg

$370
RP1075-10ug

10μg sample (liquid)

$99
RP1075-Biotin

100 μg Biotin conjugated

$570
RP1075-Cy3

100 μg Cy3 conjugated

$570
RP1075-Dylight488

100 μg Dylight488 conjugated

$570
RP1075-Dylight550

100 μg Dylight550 conjugated

$570
RP1075-Dylight594

100 μg Dylight594 conjugated

$570
RP1075-FITC

100 μg FITC conjugated

$570
RP1075-HRP

100 μg HRP conjugated

$570
RP1075-APC

100 μg APC conjugated

$670
RP1075-PE

100 μg PE conjugated

$670
RP1075-carrier-free

Carrier Free

$370

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