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Product Info Summary
| SKU: | M08199 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-LRRC32 Purified Monoclonal Antibody
SKU/Catalog Number
M08199
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LRRC32 Purified Monoclonal Antibody (Catalog# M08199). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-LRRC32 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08199)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
GARP5
Isotype
Mouse IgG1
Immunogen
Purified human sGARP protein. The mouse monoclonal antibody GARP5 recognizes GARP / LRRC32, an approximately 80 kDa glycoprotein expressed e.g. on the surface of megakaryocytes, platelets and activated Treg cells.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M08199 is reactive to LRRC32 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
72.0 kDa
Background of LRRC32
GARP (glycoprotein A repetitions predominant protein), also known as garpin or LRRC32 (leucin-rich repeat containing protein 32) is an approximately 80 kDa transmembrane glycoprotein detected on the surface of megakaryocytes, platelets, and activated Treg cells. It binds to the latency-associated peptide (LAP) domain of pro-TGF beta and regulates its storage and activation. The expression of GARP on Treg cells seems to be necessary for their suppressive functions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08199 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1-2 μg/ml; positive control: human thrombocytes, reducing conditions
Validation Images & Assay Conditions
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Anti-Hu GARP Purified (clone GARP5) works in WB application under reducing conditions.
Western blotting analysis was performed on RIPA buffer extracts of thrombocytes, leukocytes, and Jurkat cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.
Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody GARP5 (2 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml) was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.
GARP was detected at ~80 kDa in thrombocytes.
Specific Publications For Anti-LRRC32 Purified Monoclonal Antibody (M08199)
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