Anti-NDFIP1 Antibody

Product Name

Anti-NDFIP1 Antibody

View all NDFIP1 Antibodies

SKU/Catalog Number

A04644

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NDFIP1 Antibody (Catalog # A04644). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

NDFIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NDFIP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04644)

Host

Rabbit

Contents

NDFIP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NDFIP1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NDFIP1. The immunogen is located within the first 50 amino acids of NDFIP1.

Cross-reactivity

NDFIP1 antibody is predicted to not cross-react with NDFIP2.

Reactive Species

A04644 is reactive to NDFIP1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

24.9 kDa

Background of NDFIP1

The NEDD4 family-interacting protein 1 (NDFIP1) belongs to a small group of evolutionarily conserved proteins with three transmembrane domains and is an integral Golgi membrane protein. It is a potential target for ubiquitination by the Nedd4 family of proteins. NDFIP1 is strongly expressed in surviving neurons following acute cortical brain injury, and overexpression in cultured cortical neurons increased survival following growth factor starvation, suggesting that NDFIP1 may play a role in neuronal survival. NDFIP1 and the related protein NDFIP2 are thought to interact with and regulate multiple components of the EGF and PTEN/Akt signaling pathways. Recent studies suggest that NDFIP1 may also play a role in Th17 differentiation by limiting the production of proinflammatory cytokines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04644 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NDFIP1 antibody can be used for detection of NDFIP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NDFIP1

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