Anti-LRRK2 Antibody Picoband®

Lrrk2 antibody

Boster Bio Anti-LRRK2 Antibody Picoband® catalog # PB9281. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9281
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-LRRK2 Antibody Picoband®

View all Lrrk2 Antibodies

SKU/Catalog Number

PB9281

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LRRK2 Antibody Picoband® catalog # PB9281. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LRRK2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9281)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human LRRK2 recombinant protein (Position: E10-L350). Human LRRK2 shares 82% amino acid (aa) sequence identity with mouse LRRK2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9281 is reactive to LRRK2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

286 kDa

Calculated molecular weight

286103 MW

Background of Lrrk2

Leucine-rich repeat kinase 2 (LRRK2), also known as dardarin, is anenzyme that in humans is encoded by the PARK8 gene. It is a member of the leucine-rich repeat kinase family. This gene is mapped to 12q12. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons. It has been determined that LRRK2 possesses mixed-lineage kinase activity, and LRRK2 also showed autophosphorylation activity. What’s more, LRRK2 has an affinity for lipids or lipid-associated proteins and may play a role in the biogenesis or regulation of membranous intracellular structures in the brain. It is also an IFNG target gene that may be involved in signaling pathways relevant to Crohn disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9281 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: Rat Brain Tissue, Mouse Brain Tissue, Rat Liver Tissue, U87 Whole Cell, NEURO Whole Cell, A549 Whole Cell, SMMC Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For LRRK2 (Source: Uniprot.org, NCBI)

Gene Name

LRRK2

Full Name

Leucine-rich repeat serine/threonine-protein kinase 2

Weight

286103 MW

Superfamily

protein kinase superfamily

Alternative Names

Leucine-rich repeat serine/threonine-protein kinase 2;2.7.11.1;Dardarin;LRRK2;PARK8; Lrrk2|4921513O20Rik, 9330188B09Rik, AW561911, D630001M17Rik, Gm927, cI-4, cI-46|leucine-rich repeat kinase 2|leucine-rich repeat serine/threonine-protein kinase 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LRRK2, check out the LRRK2 Infographic

LRRK2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LRRK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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15 Customer Q&As for Anti-LRRK2 Antibody Picoband®

Question

Would PB9281 anti-LRRK2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

As indicated on the product datasheet, PB9281 anti-LRRK2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-14

Question

Is this PB9281 anti-LRRK2 antibody reactive to the isotypes of LRRK2?

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

The immunogen of PB9281 anti-LRRK2 antibody is E.coli-derived human LRRK2 recombinant protein (Position: E10-L350). Human LRRK2 shares 82% amino acid (aa) sequence identity with mouse LRRK2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-20

Question

Please see the WB image, lot number and protocol we used for brain using anti-LRRK2 antibody PB9281. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-18

Question

I would like using your anti-LRRK2 antibody for canonical wnt signaling pathway studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

Thanks for your inquiry. This PB9281 anti-LRRK2 antibody is validated on rat liver tissue, brain tissue, tissue lysate, mouse brain, u87 whole cell lysate, neuro whole cell lysate, a549 whole cell lysate, smmc whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-07

Question

Do you have a BSA free version of anti-LRRK2 antibody PB9281 available?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-LRRK2 antibody PB9281 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-26

Question

My colleagues were satisfied with the WB result of your anti-LRRK2 antibody. However we have observed positive staining in liver cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is LRRK2 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

From what I have seen in literature, liver does express LRRK2. Generally LRRK2 expresses in cytoplasmic vesicle. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2019-08-09

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-LRRK2 antibody PB9281. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-25

Question

We are currently using anti-LRRK2 antibody PB9281 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

The anti-LRRK2 antibody (PB9281) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-21

Question

I have a question about product PB9281, anti-LRRK2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-12-05

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9281 anti-LRRK2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-12-05

Question

Is a blocking peptide available for product anti-LRRK2 antibody (PB9281)?

Verified Customer

Verified customer

Asked: 2018-11-21

Answer

We do provide the blocking peptide for product anti-LRRK2 antibody (PB9281). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-11-21

Question

I am looking for to test anti-LRRK2 antibody PB9281 on rat brain for research purposes, then I may be interested in using anti-LRRK2 antibody PB9281 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-11-27

Answer

The products we sell, including anti-LRRK2 antibody PB9281, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-11-27

Question

Does anti-LRRK2 antibody PB9281 work for WB with brain?

Verified Customer

Verified customer

Asked: 2017-10-30

Answer

According to the expression profile of brain, LRRK2 is highly expressed in brain. So, it is likely that anti-LRRK2 antibody PB9281 will work for WB with brain.

Boster Scientific Support

Answered: 2017-10-30

Question

We have observed staining in rat testis. Are there any suggestions? Is anti-LRRK2 antibody supposed to stain testis positively?

S. Miller

Verified customer

Asked: 2016-01-15

Answer

According to literature testis does express LRRK2. According to Uniprot.org, LRRK2 is expressed in visceral pleura, brain, testis, liver, among other tissues. Regarding which tissues have LRRK2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15541309
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2016-01-15

Question

I was wanting to use your anti-LRRK2 antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?

A. Carter

Verified customer

Asked: 2015-11-19

Answer

It shows on the product datasheet, PB9281 anti-LRRK2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-11-19

Question

I see that the anti-LRRK2 antibody PB9281 works with WB, what is the protocol used to produce the result images on the product page?

K. Yang

Verified customer

Asked: 2013-04-29

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-04-29

Order DetailsPrice
PB9281

100μg

$370
PB9281-10ug

10μg sample (liquid)

$99
PB9281-Biotin

100 μg Biotin conjugated

$570
PB9281-Cy3

100 μg Cy3 conjugated

$570
PB9281-Dylight488

100 μg Dylight488 conjugated

$570
PB9281-Dylight550

100 μg Dylight550 conjugated

$570
PB9281-Dylight594

100 μg Dylight594 conjugated

$570
PB9281-FITC

100 μg FITC conjugated

$570
PB9281-HRP

100 μg HRP conjugated

$570
PB9281-APC

100 μg APC conjugated

$670
PB9281-PE

100 μg PE conjugated

$670
PB9281-iFluor647

100 μg iFluor647 conjugated

$670
PB9281-carrier-free

Carrier Free

$370

More conjugates/formats

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PB9281
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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