Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband™

Product Name

Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband™

View all HSPG2 Antibodies

SKU/Catalog Number

PB9277

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband™ catalog # PB9277. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9277)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HSPG2 recombinant protein (Position: F524-K701). Human HSPG2 shares 86% amino acid (aa) sequence identity with mouse HSPG2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9277 is reactive to HSPG2 in Human

Applications

PB9277 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

469 kDa

Calculated molecular weight

468.83kDa

Background of HSPG2

Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Anti-HSPG2 Picoband antibody, PB9277,IHC(P)
IHC(P): Human Lung Cancer Tissue

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Figure 1. Western blot analysis of HSPG2 using anti-HSPG2 antibody (PB9277).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPG2 antigen affinity purified polyclonal antibody (Catalog # PB9277) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPG2 at approximately 469KD. The expected band size for HSPG2 is at 469KD.

Gene/Protein Information For HSPG2 (Source: Uniprot.org, NCBI)

Alternative Names

basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); Endorepellin; heparan sulfate proteoglycan 2; HSPG; HSPG2; perlecan proteoglycan; Perlecan; PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia); PRCAN; SJA; SJS; SJS1 HSPG2 HSPG, PLC, PRCAN, SJA, SJS, SJS1 heparan sulfate proteoglycan 2 basement membrane-specific heparan sulfate proteoglycan core protein|endorepellin (domain V region)|perlecan proteoglycan

For more info on HSPG2, check out the HSPG2 Infographic

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In this infographic, you will see the following information for HSPG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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