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Product Info Summary
| SKU: | PB9277 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband®
SKU/Catalog Number
PB9277
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband® catalog # PB9277. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9277)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HSPG2 recombinant protein (Position: F524-K701). Human HSPG2 shares 86% amino acid (aa) sequence identity with mouse HSPG2.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9277 is reactive to HSPG2 in Human
Observed Molecular Weight
469 kDa
Calculated molecular weight
468.8 kDa
Background of HSPG2
Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9277 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Caco-2 whole cell, human A549 whole cell
IHC: Human Lung Cancer Tissue
Validation Images & Assay Conditions
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Anti-HSPG2 Picoband antibody, PB9277,IHC(P)
IHC(P): Human Lung Cancer Tissue
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Western blot analysis of HSPG2 using anti-HSPG2 antibody (PB9277).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPG2 antigen affinity purified polyclonal antibody (Catalog # PB9277) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPG2 at approximately 469KD. The expected band size for HSPG2 is at 469KD.
Specific Publications For Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband® (PB9277)
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Customer Q&As
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2 Customer Q&As for Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband®
Question
We are currently using anti-Heparan Sulfate Proteoglycan 2/HSPG2 antibody PB9277 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
The anti-Heparan Sulfate Proteoglycan 2/HSPG2 antibody (PB9277) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-21
Question
On your website, the HSPG2 gel band for your western blot appears at 69 kDa. However, the band in your retest is 469 kDa. Is there a typo somewhere?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
No. The expected MW is 469 kDa: details can be found at https://www.uniprot.org/uniprot/P98160.
Boster Scientific Support
Answered: 2017-10-12
