Anti-Lsk MATK Monoclonal Antibody

MATK antibody

Boster Bio Anti-Lsk MATK Monoclonal Antibody catalog # A02050. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A02050
Size: 100μl
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, WB

Product Name

Anti-Lsk MATK Monoclonal Antibody

View all MATK Antibodies

SKU/Catalog Number

A02050

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Lsk MATK Monoclonal Antibody catalog # A02050. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lsk MATK Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02050)

Host

Mouse

Contents

Antibody are purified by protein G affinity chromatography. Liquid in PBS containing 50% glycerol and 0.03% sodium azide.

Clonality

Monoclonal

Clone Number

9D7

Isotype

IgG

Immunogen

Purified recombinant fragment of human Lsk expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02050 is reactive to MATK in Human

Applications

A02050 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

56.469kDa

Background of MATK

MEK6 is a member of MAPKK protein kinase family. By using degenerate oligonucleotide primers from the conserved kinase domains of MKK3 and MKK4 two human cDNAs and 1 murine cDNA encoding closely related proteins of the MKK family were cloned. The two human clones appear to be different isoforms of the same gene generated by differential splicing: the shorter clone was designated MKK6, encodes a 278-amino acid protein, while the longer clone, designated MKK6b, encodes a 334-amino acid protein. MKK6 is about 80% identical to MKK3 and 40% identical to MKK4. 1.7-kb human MKK6 transcript is highly expressed in skeletal muscle, while an MKK6b-specific probe detected mRNA bands of 1.8, 2.4, and 4.5 kb that are enriched in heart, skeletal muscle, pancreas and liver. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase . MEK6 phosphorylates and activates p38 in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAPK mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.

Wang W, et al. (2002) Mol Cell Biol ; 22(10): 3389-403.
Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
FC, 1:200-1:400

Validation Images & Assay Conditions

Gene/Protein Information For MATK (Source: Uniprot.org, NCBI)

Gene Name

MATK

Full Name

Megakaryocyte-associated tyrosine-protein kinase

Weight

56.469kDa

Superfamily

protein kinase superfamily

Alternative Names

CHK; Csk-type protein tyrosine kinase; CTKEC 2.7.10.2; DKFZp434N1212; EC 2.7.10; Hematopoietic consensus tyrosine-lacking kinase; HHYLTK; hydroxyaryl-protein kinase; HYL tyrosine kinase; HYLCsk-homologous kinase; HYLTK; leukocyte carboxyl-terminal src kinase related; Lsk; megakaryocyte-associated tyrosine kinase; megakaryocyte-associated tyrosine-protein kinase; MGC1708; MGC2101; Protein kinase HYL; tyrosine kinase MATK; Tyrosine-protein kinase CTK; tyrosylprotein kinase MATK CHK, CTK, HHYLTK, HYL, HYLTK, Lsk megakaryocyte-associated tyrosine kinase megakaryocyte-associated tyrosine-protein kinase|CSK homologous kinase|Csk-homologous kinase|Csk-type protein tyrosine kinase|HYL tyrosine kinase|hematopoietic consensus tyrosine-lacking kinase|hydroxyaryl-protein kinase|leukocyte carboxyl-terminal src kinase related|protein kinase HYL|tyrosine kinase MATK|tyrosine-protein kinase CTK|tyrosylprotein kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MATK, check out the MATK Infographic

MATK infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MATK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Lsk MATK Monoclonal Antibody

Question

We are currently using anti-Lsk Monoclonal antibody A02050 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

The anti-Lsk Monoclonal antibody (A02050) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-04

Question

I see that the anti-Lsk Monoclonal antibody A02050 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-04-17

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-04-17

Question

Would A02050 anti-Lsk Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-11-09

Answer

As indicated on the product datasheet, A02050 anti-Lsk Monoclonal antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-11-09

Question

Would anti-Lsk Monoclonal antibody A02050 work on monkey Flow Cytometry with testis?

C. Singh

Verified customer

Asked: 2015-08-20

Answer

Our lab technicians have not validated anti-Lsk Monoclonal antibody A02050 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Lsk Monoclonal antibody A02050 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey testis in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-08-20

Question

I was wanting to use your anti-Lsk Monoclonal antibody for Flow Cytometry for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?

B. Krishna

Verified customer

Asked: 2014-10-20

Answer

It shows on the product datasheet, A02050 anti-Lsk Monoclonal antibody has been tested for Flow Cytometry, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-10-20

Order DetailsPrice
A02050

100uL

$399

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