Anti-TCP1 theta/CCT8 Antibody Picoband™

CCT8 antibody

Boster Bio Anti-TCP1 theta/CCT8 Antibody Picoband™ catalog # PB9993. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9993
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

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Product Name

Anti-TCP1 theta/CCT8 Antibody Picoband™

View all CCT8 Antibodies

SKU/Catalog Number

PB9993

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TCP1 theta/CCT8 Antibody Picoband™ catalog # PB9993. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TCP1 theta/CCT8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9993)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CCT8 recombinant protein (Position: K421-D548). Human CCT8 shares 98.4% amino acid (aa) sequence identity with mouse CCT8.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9993 is reactive to CCT8 in Human, Mouse, Rat

Applications

PB9993 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

59.621kDa

Background of CCT8

T-complex protein 1 subunit theta is a protein that in humans is encoded by the CCT8 gene. This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CCT8 (Source: Uniprot.org, NCBI)

Gene Name

CCT8

Full Name

T-complex protein 1 subunit theta

Weight

59.621kDa

Superfamily

TCP-1 chaperonin family

Alternative Names

C21orf112; Cctq; CCT-theta; chaperonin containing TCP1, subunit 8 (theta); chromosome 21 open reading frame 112; D21S246; KIAA0002; PRED71; T-complex protein 1 subunit theta; T-complex protein 1, theta subunit, 10Renal carcinoma antigen NY-REN-15; TCP-1-theta CCT8 C21orf112, Cctq, D21S246, PRED71 chaperonin containing TCP1 subunit 8 T-complex protein 1 subunit theta|CCT-theta|TCP-1-theta|chaperonin containing T-complex polypeptide 1 subunit 8|chaperonin containing TCP1, subunit 8 (theta)|renal carcinoma antigen NY-REN-15

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CCT8, check out the CCT8 Infographic

CCT8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCT8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-TCP1 theta/CCT8 Antibody Picoband™

Question

Can you help my question with product PB9993, anti-TCP1 theta/CCT8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9993 anti-TCP1 theta/CCT8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-10

Question

We are currently using anti-TCP1 theta/CCT8 antibody PB9993 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

The anti-TCP1 theta/CCT8 antibody (PB9993) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-28

Question

Does anti-TCP1 theta/CCT8 antibody PB9993 work for WB with cervix carcinoma?

G. Jha

Verified customer

Asked: 2018-11-05

Answer

According to the expression profile of cervix carcinoma, CCT8 is highly expressed in cervix carcinoma. So, it is likely that anti-TCP1 theta/CCT8 antibody PB9993 will work for WB with cervix carcinoma.

Boster Scientific Support

Answered: 2018-11-05

Question

Is this PB9993 anti-TCP1 theta/CCT8 antibody reactive to the isotypes of CCT8?

Verified Customer

Verified customer

Asked: 2018-05-02

Answer

The immunogen of PB9993 anti-TCP1 theta/CCT8 antibody is E.coli-derived human CCT8 recombinant protein (Position: K421-D548). Human CCT8 shares 98.4% amino acid (aa) sequence identity with mouse CCT8. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-05-02

Question

My question regards to test anti-TCP1 theta/CCT8 antibody PB9993 on human cervix carcinoma for research purposes, then I may be interested in using anti-TCP1 theta/CCT8 antibody PB9993 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

D. Zhao

Verified customer

Asked: 2016-04-07

Answer

The products we sell, including anti-TCP1 theta/CCT8 antibody PB9993, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-04-07

Order DetailsPrice
PB9993

100μg

$370
PB9993-10ug

10μg sample (liquid)

$99
PB9993-Biotin

100 μg Biotin conjugated

$570
PB9993-Cy3

100 μg Cy3 conjugated

$570
PB9993-Dylight488

100 μg Dylight488 conjugated

$570
PB9993-Dylight550

100 μg Dylight550 conjugated

$570
PB9993-Dylight594

100 μg Dylight594 conjugated

$570
PB9993-FITC

100 μg FITC conjugated

$570
PB9993-HRP

100 μg HRP conjugated

$570
PB9993-APC

100 μg APC conjugated

$670
PB9993-PE

100 μg PE conjugated

$670
PB9993-carrier-free

Carrier Free

$370

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PB9993
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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