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LSM8 antibody (M10947) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of JK cells using anti-LSM8 antibody (M10947).
Overlay histogram showing JK cells stained with M10947 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-LSM8 Antibody (M10947, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing JK cells stained with M10947 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-LSM8 Antibody (M10947, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-LSM8 Antibody Picoband® (monoclonal, 6B11)
LSM8 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
LSM8 antibody was positively detected by WB in:
human HL-60 whole cell, human Jurkat whole cell, human THP-1 whole, human K562 whole cell, rat testis tissue, rat pancrease tissue, mouse testis tissue, mouse pancrease tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
LSM8 antibody was positively detected by IHC in:
human renal clear cell carcinoma tissue, human colonic adenocarcinoma tissue, human placenta tissue, human spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
LSM8 antibody was positively detected by ICC/IF in:
Hela cell
LSM8 antibody was positively detected by FC in:
JK cell, RH35 cell, RAW2647 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-LSM8 Antibody Picoband® (monoclonal, 6B11)
View all LSM8 Antibodies
View all LSM8 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6B11
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human LSM8 recombinant protein (Position: M1-H96).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
16 kDa
Calculated Molecular Weight
10.4 kDa
Background of LSM8
U6 snRNA-associated Sm-like protein LSm8 is a protein that in humans is encoded by the LSM8 gene. This gene encodes a member of the like-Sm family of proteins. The encoded protein consists of a closed barrel shape, made up of five anti-parallel beta strands and an alpha helix. This protein partners with six paralogs to form a heteroheptameric ring which transiently binds U6 small nuclear RNAs and is involved in the general maturation of RNA in the nucleus.
Cite This Product
Anti-LSM8 Antibody Picoband® (monoclonal, 6B11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10947)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Zebrafish
92%
High Homology
Dog
91%
High Homology
Drosophila
68%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for LSM8 antibody
Immunohistochemistry (IHC) protocol for LSM8 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for LSM8 antibody
Flow Cytometry (FC) protocol for LSM8 antibody
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Product Promise
We promise Anti-LSM8 Antibody Picoband® (monoclonal, 6B11) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M10947
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026