Anti-MAP1LC3C Antibody

Product Name

Anti-MAP1LC3C Antibody

View all MAP1LC3C Antibodies

SKU/Catalog Number

A08533

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-MAP1LC3C Antibody (Catalog # A08533). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

MAP1LC3C antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-MAP1LC3C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08533)

Host

Rabbit

Contents

MAP1LC3C antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

MAP1LC3C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human MAP1LC3C. The immunogen is located within the last 50 amino acids of MAP1LC3C.

Cross-reactivity

MAP1LC3C antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3C are known to exist. MAP1LC3C antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3B.

Reactive Species

A08533 is reactive to MAP1LC3C in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

16.9 kDa

Background of MAP1LC3C

Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3C belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2). MAP1LC3C is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3C, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08533 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MAP1LC3C antibody can be used for detection of MAP1LC3C by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information MAP1LC3C

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