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Product Info Summary
| SKU: | M03011-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-MAPK6 Mouse Monoclonal Antibody [Clone ID: OTI5E1]
SKU/Catalog Number
M03011-2
Size
100 µl
Description
Boster Bio Anti-ERK3 mouse monoclonal antibody, clone OTI5E1 (formerly 5E1). Catalog# M03011-2. Tested in IF, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-MAPK6 Mouse Monoclonal Antibody [Clone ID: OTI5E1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03011-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI5E1
Isotype
IgG2a
Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3
Reactive Species
M03011-2 is reactive to MAPK6 in Human, Mouse, Rat
Calculated molecular weight
82.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03011-2 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IF 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.
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Western blot analysis of extracts (10ug) from 1 cell line by using anti-MAPK6 monoclonal antibody at 1:200 dilution.
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Anti-MAPK6 mouse monoclonal antibody (M03011-2) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6
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Immunofluorescent staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody (M03011-2).
Specific Publications For Anti-MAPK6 Mouse Monoclonal Antibody [Clone ID: OTI5E1] (M03011-2)
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