Anti-CUEDC2 Antibody

Product Name

Anti-CUEDC2 Antibody

View all Cuedc2 Antibodies

SKU/Catalog Number

PA1962

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CUEDC2 Antibody catalog # PA1962. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CUEDC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1962)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CUEDC2, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1962 is reactive to Cuedc2 in Human, Mouse, Rat

Applications

PA1962 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

32 kDa, 19 kDa

Calculated molecular weight

31.852kDa

Background of Cuedc2

CUEDC2 (Cue Domain-Containing Protein 2) is involved in ubiquitin-and proteasome-mediated degradation of progesterone receptor (PR, or PGR) and estrogen receptor (ER)-alpha. Hartz (2011) mapped the CUEDC2 gene to chromosome 10q24.32 based on an alignment of the CUEDC2 sequence with the genomic sequence (GRCh37). Using coimmunoprecipitation analysis and protein pull-down assays, Zhang et al. (2007) confirmed that CUEDC2 interacted with the PRB isoform of PR. Mutation analysis revealed that the IF domain of PRB and the CUE domain of CUEDC2 were required for the interaction. CUEDC2 reduced PRB protein content and promoted progesterone-induced PRB degradation via the ubiquitin-proteasome pathway.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Anti-CUEDC2 antibody, PA1962, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human CuEDC2, 28.9KD (162aa tag+ N197-H287)
Lane 1: Recombinant Human CuEDC2 Protein 10ng
Lane 2: Recombinant Human CuEDC2 Protein 5ng
Lane 3: Recombinant Human CuEDC2 Protein 2.5ng
Lane 4: Recombinant Human CuEDC2 Protein 1.25ng

Click image to see more details

Anti-CUEDC2 antibody, PA1962, IHC(P)
IHC(P): Human Thyroid Cancer Tissue

Gene/Protein Information For Cuedc2 (Source: Uniprot.org, NCBI)

Alternative Names

bA18I14.5; C10orf66; chromosome 10 open reading frame 66; CUE domain containing 2; CUE domain-containing protein 2; MGC2491 Cuedc2|3010002G01Rik|CUE domain containing 2|CUE domain-containing protein 2

For more info on Cuedc2, check out the Cuedc2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cuedc2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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