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- Table of Contents
CGAS antibody (A31676-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of THP-1 cells using anti-CGAS antibody (A31676-1).
Overlay histogram showing THP-1 cells stained with A31676-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CGAS Antibody (A31676-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with A31676-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CGAS Antibody (A31676-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CGAS Antibody Picoband®
CGAS antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
CGAS antibody was positively detected by WB in:
human Hela whole cell, human Caco-2 whole cell, human A431 whole cell, human Hela- WT whole cell, human Hela-CGAS KO whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CGAS antibody was positively detected by IHC in:
human tonsil tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CGAS antibody was positively detected by ICC/IF in:
CACO-2 cell
CGAS antibody was positively detected by FC in:
THP-1 cell, U937 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CGAS Antibody Picoband®
View all CGAS Antibodies
View all CGAS Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CGAS recombinant protein (Position: L208-F522).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
62 kDa
Calculated Molecular Weight
58.8 kDa
Background of CGAS
cGAS (cyclic GMP-AMP synthase), also known as MB21D1 (Mab-21 domain containing 1), h-cGAS or C6orf150, is a 522 amino acid cytoplasmic nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. cGAS is suggested to have antiviral activity by acting as a key cytosolic DNA sensor. cGAS binds to cytosolic DNA, which leads to cGAMP synthesis and activation of TMEM173, thereby trigger type-I interferon production. Expressed in monocytic cell line THP1, cGAS exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 6q13.
Cite This Product
Anti-CGAS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31676-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
90%
High Homology
Sheep
79%
Moderate Homology
Cow
74%
Moderate Homology
Mouse
72%
Moderate Homology
Rat
71%
Moderate Homology
Dog
58%
Low homology
Zebrafish
43%
Low homology
Drosophila
23%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CGAS antibody
Immunohistochemistry (IHC) protocol for CGAS antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CGAS antibody
Flow Cytometry (FC) protocol for CGAS antibody
Publications
Application:
Species:
Electroacupuncture protects gastric Cajal cells by reducing macrophage pyroptosis in diabetic gastroparesis
Published: 2026-02-15
Journal: World Journal of Diabetes
Application: WB
Impact Factor: 4.60
Species: Rat
PMID: 41696103
cGAS mRNA-based Immune Agonist Promotes Vaccine Responses and Antitumor Immunity
Published: 2025-03-11
Journal: Cancer Immunology Research
Application: WB
Impact Factor: 8.20
Species: Human
PMID: 40067177
Hydrogel loaded with cerium-manganese nanoparticles and nerve growth factor enhances spinal cord injury repair by modulating immune microenvironment and promoting neuronal regeneration
Published: 2025-01-20
Journal: JOURNAL OF NANOBIOTECHNOLOGY
Application: WB
Impact Factor: 12.60
Species: Mouse
PMID: 39833803
Inhibition of inflammation and apoptosis through the cyclic GMP-AMP synthase-stimulator of interferon genes pathway by stress granules after ALKBH5 demethylase activation during diabetic myocardial ischaemia-reperfusion injury
Published: 2024-07-11
Journal: DIABETES OBESITY & METABOLISM
Application: WB
Impact Factor: 5.40
Species: Rat
PMID: 38988216
The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson’s disease
Published: 2024-06-03
Journal: Neural Regeneration Research
Application: WB
Impact Factor: 5.90
Species: Mouse
PMID: 39359093
JMJD2A promotes the development of castration-resistant prostate cancer by activating androgen receptor enhancer and inhibiting the cGAS-STING pathway
Published: 2024-05-31
Journal: MOLECULAR CARCINOGENESIS
Impact Factor: 3.00
PMID: 38818897
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Product Promise
We promise Anti-CGAS Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A31676-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026