Cyclic GMP-AMP synthase Antibodies

AND CGAS ELISA kits, Cyclic GMP-AMP synthase Proteins

Many biological assays use antibodies to detect Cyclic GMP-AMP synthase, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Cyclic GMP-AMP synthase in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Cyclic GMP-AMP synthase antibodies...

We validate the specificity of these antibodies to Cyclic GMP-AMP synthase by testing them on tissues known to express CGAS positively and negatively. Browse below to find the CGAS antibody that suites your experiment. We have 5 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for Cyclic GMP-AMP synthase? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the CGAS Western Blot Guide

CGAS Infographic by Boster Bio

All CGAS Products

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Anti-CGAS Antibody Picoband®

6 Citations

IF analysis of CGAS using anti-CGAS antibody (A31676-1)

Flow Cytometry analysis of THP-1 cells using anti-CGAS antibody (A31676-1)

Flow Cytometry analysis of U937 cells using anti-CGAS antibody (A31676-1)

Human

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A31676-1
100 μg/vial

Anti-cGAS Antibody

1 Citations

IHC analysis of cGAS using anti-cGAS antibody (A31676-2)

Human, Mouse

ELISA, IF, IHC, ICC, WB

$370
Cat # A31676-2
100 μg/vial

Anti-MB21D1 CGAS Antibody

1 Citations

Western blot analysis of extracts of various cell lines, using MB21D1 antibody

Human

ELISA, IHC, WB

$405
Cat # A31676
100ul

Anti-cGAS Rabbit Monoclonal Antibody

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-cGAS antibody

Human

ELISA, IP, IF, IHC, WB

$146
Cat # M00709-1
20μL, 40μL, 100μL, 200μL

C6orf150 (MB21D1) (NM_138441) Human Over-expression Lysate

Human

$960
Cat # LS115004
20 µg, 100 µg

CGAS Overview

Facts about Cyclic GMP-AMP synthase.

CGAS 3D structure. Source: alphafold

Cyclic GMP-AMP synthase (CGAS)

Catalysis involves the creation of a 2',5' phosphodiester linkage at the GpA step and the creation of a 3',5' phosphodiester linkage at the ApG step, generating c[G(2',5')pA(3',5')p] (PubMed:28363908, PubMed:28214358). Acts as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune reactions (PubMed:28363908).

Binds cytosolic DNA directly, resulting in activation and synthesis of cGAMP, another messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production (PubMed:28363908, PubMed:28314590). Also functions as an innate immune sensor of infection by retroviruses, such as HIV-1, by detecting the presence of reverse-transcribed DNA in the cytosol (PubMed:23929945).

Gene Information

Human
Gene Name:	CGAS
Uniprot:	Q8N884
Entrez:	115004

Family

Belongs to:

mab-21 family

Alternative Names

Cyclic GMP-AMP synthase

Molecular Weight

Mass (kDA):
58.814 kDA

Genomic Context


Human

Location:	6q13
Sequence:	6; NC_000006.12 (73423711..73452297, complement)

Tissue Specificity

Expressed in the monocytic cell line THP1.

Subcellular Localizations

Cell membrane; Peripheral membrane protein. Cytoplasm, cytosol. Nucleus. In resting conditions, localizes at the cell membrane as a peripheral membrane protein by binding to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (PubMed:30827685). Localization at the cell membrane is required to limit the recognition of self-DNA (PubMed:30827685). Following detection of double-stranded DNA (dsDNA), released from the cell membrane into the cytosol in order to signal (PubMed:30827685). Upon transfection with dsDNA forms punctate structures that co-localize with DNA and Beclin-1 (BECN1) (PubMed:26

PTMs

Acetylation

Isopeptide bond

Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CGAS

References

PMID: 23258413 by Sun L., et al. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway.

PMID: 21478870 by Schoggins J.W., et al. A diverse range of gene products are effectors of the type I interferon antiviral response.