Anti-MBD1 Antibody (Monoclonal, 35M14)

Product Name

Anti-MBD1 Antibody (Monoclonal, 35M14)

View all Mbd1 Antibodies

SKU/Catalog Number

M02336-2

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-MBD1 Antibody (Monoclonal, 35M14) catalog # M02336-2. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MBD1 Antibody (Monoclonal, 35M14) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02336-2)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

35M14

Immunogen

Recombinant protein within mouse MBD1 aa 3-198.

Reactive Species

M02336-2 is reactive to Mbd1 in Mouse, Rat

Calculated molecular weight

70.0 kDa

Background of Mbd1

MBD1(Methyl-CpG-Binding Domain Protein 1), also known as PCM1 or CXXC3, is a protein that in humans is encoded by the MBD1 gene. Using PCR on a hybrid panel and FISH, Hendrich et al.(1999) mapped the MBD1 gene to chromosome 18q21, 2.1 cM distal to MBD2. Using yeast 2-hybrid analysis, reciprocal immunoprecipitation analysis, and protein pull-down assays, Fujita et al.(2003) showed that MBD1 interacted directly with MCAF. Deletion analysis revealed that the C-terminal transcriptional repressor domain(TRD) of MBD1 interacted with a conserved C-terminal domain of MCAF. Reporter gene assays showed that MCAF increased the repressive function of the isolated TRD of MBD1 against SP1. Chromatin immunoprecipitation analysis revealed that MBD1 linked MCAF to methylated promoters. Uchimura et al.(2006) found that MBD1 was multiply sumoylated in HeLa cells. Sumoylation did not alter the intracellular localization of MBD1 at nuclear foci in C-33A human cervical cancer cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02336-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunocytochemistry/Immunofluorescence, 1:50-200
Flow Cytometry (Fixed), 1:50-200

Validation Images & Assay Conditions

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See full target information Mbd1

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