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Product Info Summary
| SKU: | A07019-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MC5-R Antibody
SKU/Catalog Number
A07019-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MC5-R Antibody catalog # A07019-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MC5-R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07019-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MC5R. AA range:271-320
Reactive Species
A07019-1 is reactive to MC5R in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
36.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07019-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Immunofluorescence analysis of MCF7 cells, using MC5R Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of 3T3 cells using MC5-R Polyclonal Antibody
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Western blot analysis of lysates from K562 cells, using MC5R Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using MC5R antibody.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-MC5-R Antibody (A07019-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-MC5-R Antibody
Question
Is this A07019-1 anti-MC5-R antibody reactive to the isotypes of MC5R?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
The immunogen of A07019-1 anti-MC5-R antibody is Synthesized peptide derived from the C-terminal region of human MC5-R. at AA rangle: 240-320. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-10
Question
Do you have a BSA free version of anti-MC5-R antibody A07019-1 available?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MC5-R antibody A07019-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-19
Question
Will anti-MC5-R antibody A07019-1 work for WB with neocortex?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
According to the expression profile of neocortex, MC5R is highly expressed in neocortex. So, it is likely that anti-MC5-R antibody A07019-1 will work for WB with neocortex.
Boster Scientific Support
Answered: 2019-01-23
Question
Is a blocking peptide available for product anti-MC5-R antibody (A07019-1)?
Verified Customer
Verified customer
Asked: 2018-10-24
Answer
We do provide the blocking peptide for product anti-MC5-R antibody (A07019-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-10-24
Question
My question regarding product A07019-1, anti-MC5-R antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07019-1 anti-MC5-R antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-01
Question
We are currently using anti-MC5-R antibody A07019-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-23
Answer
The anti-MC5-R antibody (A07019-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-23
Question
Please see the WB image, lot number and protocol we used for neocortex using anti-MC5-R antibody A07019-1. Please let me know if you require anything else.
S. Li
Verified customer
Asked: 2016-08-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-01
