Click image to see more details
Product Info Summary
| SKU: | PA1378-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MDM2 Antibody Picoband®
SKU/Catalog Number
PA1378-1
BA3612-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MDM2 Antibody catalog # PA1378-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MDM2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1378-1)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MDM2, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1378-1 is reactive to MDM2 in Human
Observed Molecular Weight
90 kDa
Calculated molecular weight
55.2 kDa
Background of MDM2
Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al. (1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1378-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: Human Caco-2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of MDM2 using anti-MDM2 antibody (PA1378-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Human Caco-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDM2 antigen affinity purified polyclonal antibody (Catalog # PA1378-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDM2 at approximately 90KD. The expected band size for MDM2 is at 55KD.
Click image to see more details
Western blot analysis of MDM2 using anti-MDM2 antibody (PA1378-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with non-fat dry milk (5%) in TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDM2 antibody (PA1378-1) at 8µL in 6 mLs (not because it needed that concentration but because that was the size of my frozen aliquots) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Cell Signaling Technology's 7074s murine anti-rabbit conformation specific antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an SuperSignal West Pico Plus with a BioRad imager. A specific band was detected for MDM2 at approximately 55 kDa. The expected band size for MDM2 is at 55 kDa.
Specific Publications For Anti-MDM2 Antibody Picoband® (PA1378-1)
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Customer Reviews
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1 Reviews For Anti-MDM2 Antibody Picoband®
Boster's Anti-MDM2 Antibody Worked!--Stacy Wilson, Biology, REVOLUTION Medicines, Inc., SRA
Excellent
Source: Biocompare.com
| SKU | PA1378-1 |
|---|---|
| Applications | Western Blot |
| Sample | MDA-MB-468 and HEK293E cell lysates |
| Detection | SuperSignal West Pico Plus with a BioRad imager |
"I've been looking at downstream products of AKT and mTOR transcriptional regulation. I tried several anti-MDM2 antibodies from other companies with either ambiguous or negative results (in Western blots of human cancer cell line lysates) and Boster's is the only one that gave me strong bands at the two expected sizes. I would recommend this product. I would absolutely buy it again."
Stacy Wilson
Verified customer
Submitted 2017-08-21
Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
15 Customer Q&As for Anti-MDM2 Antibody Picoband®
Question
Is a blocking peptide available for product anti-MDM2 antibody (PA1378-1)?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
We do provide the blocking peptide for product anti-MDM2 antibody (PA1378-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-07
Question
Our team were content with the WB result of your anti-MDM2 antibody. However we have been able to see positive staining in rhabdomyosarcoma nucleus using this antibody. Is that expected? Could you tell me where is MDM2 supposed to be expressed?
M. Evans
Verified customer
Asked: 2020-01-21
Answer
According to literature, rhabdomyosarcoma does express MDM2. Generally MDM2 expresses in nucleus, nucleoplasm. cytoplasm. nucleus,. Regarding which tissues have MDM2 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1614537
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Ovarian carcinoma, Pubmed ID: 8705862
Rhabdomyosarcoma, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-21
Question
Will anti-MDM2 antibody PA1378-1 work for WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
According to the expression profile of erythroleukemia, MDM2 is highly expressed in erythroleukemia. So, it is likely that anti-MDM2 antibody PA1378-1 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2020-01-16
Question
Do you have a BSA free version of anti-MDM2 antibody PA1378-1 available?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MDM2 antibody PA1378-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-31
Question
I was wanting to use your anti-MDM2 antibody for WB for human erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
As indicated on the product datasheet, PA1378-1 anti-MDM2 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-17
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-MDM2 antibody PA1378-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-08
Question
Does anti-MDM2 antibody PA1378-1 work on monkey WB with colon?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Our lab technicians have not tested anti-MDM2 antibody PA1378-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-MDM2 antibody PA1378-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
We have seen staining in human colon. Do you have any suggestions? Is anti-MDM2 antibody supposed to stain colon positively?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
Based on literature colon does express MDM2. Based on Uniprot.org, MDM2 is expressed in oviduct epithelium, colon, ovarian carcinoma, tongue, rhabdomyosarcoma, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have MDM2 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 1614537
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Ovarian carcinoma, Pubmed ID: 8705862
Rhabdomyosarcoma, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-06-05
Question
Please see the WB image, lot number and protocol we used for erythroleukemia using anti-MDM2 antibody PA1378-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-23
Question
Will PA1378-1 anti-MDM2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Jones
Verified customer
Asked: 2018-05-23
Answer
You can see on the product datasheet, PA1378-1 anti-MDM2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-23
Question
I was wanting to use to test anti-MDM2 antibody PA1378-1 on human erythroleukemia for research purposes, then I may be interested in using anti-MDM2 antibody PA1378-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
The products we sell, including anti-MDM2 antibody PA1378-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-02
Question
Is this PA1378-1 anti-MDM2 antibody reactive to the isotypes of MDM2?
H. Lewis
Verified customer
Asked: 2017-09-01
Answer
The immunogen of PA1378-1 anti-MDM2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MDM2(343-358aa EKAKLENSTQAEEGFD), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-01
Question
I see that the anti-MDM2 antibody PA1378-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-04
Question
I have a question about product PA1378-1, anti-MDM2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Dhar
Verified customer
Asked: 2017-03-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1378-1 anti-MDM2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-03-03
Question
We are currently using anti-MDM2 antibody PA1378-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
J. Wu
Verified customer
Asked: 2013-11-15
Answer
The anti-MDM2 antibody (PA1378-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-15
