Anti-P53 Antibody

Product Name

Anti-P53 Antibody

View all TP53 Antibodies

SKU/Catalog Number

M00001

Size

50 µl

Description

Boster Bio Anti-P53 Antibody (Catalog # M00001). Tested in WB, IHC-P application(s). This antibody reacts with Human, African green monkey.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-P53 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00001)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1711CT184.18.1

Isotype

IgG1,k

Immunogen

This P53 antibody is generated from a mouse immunized with a recombinant protein conjugated synthetic peptide between 1-393 amino acids from human P53.

Reactive Species

M00001 is reactive to TP53 in African green monkey, Human

Calculated molecular weight

43.7 kDa

Background of TP53

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00001 is guaranteed for IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC: 1:400
WB: 1:2000

Validation Images & Assay Conditions

See full target information TP53

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