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MMP1 antibody (A00733-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 17 Publications

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a00733 1 mmp1 primary antibodies fcm testing 6

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Flow Cytometry analysis of PC-3 cells using anti-MMP1 antibody (A00733-1).
Overlay histogram showing PC-3 cells stained with A00733-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MMP1 Antibody (A00733-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-MMP1 Antibody Picoband®

MMP1 antibody

17 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

MMP1 antibody was positively detected by WB in:

human placenta tissue, human CACO-2 whole cell, human K562 whole cell, human U20S whole cell, human MCF-7 whole cell, rat RH35 whole cell, mouse HEPA1-6 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

MMP1 antibody was positively detected by IHC in:

human mammary cancer tissue, human placenta tissue, human intestinal cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

MMP1 antibody was positively detected by ICC/IF in:

U20S cell

MMP1 antibody was positively detected by FC in:

PC-3 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Product Information

Product Name

Anti-MMP1 Antibody Picoband®
View all MMP1 Antibodies

Form

Lyophilized

Uniprot

P03956

Gene ID

4312

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MMP1 recombinant protein (Position: D124-N469).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

54 kDa

Calculated Molecular Weight

54.0 kDa

Background of MMP1

Matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase, is an enzyme that in humans is encoded by the MMP1 gene. MMP-1 was the first vertebrate collagenase both purified to homogeneity as a protein, and cloned as a cDNA. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. It is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.

Cite This Product

Anti-MMP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00733-1)

Protocols

Western Blot protocol for MMP1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for MMP1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MMP1 antibody

Review Protocol

Flow Cytometry (FC) protocol for MMP1 antibody

Review Protocol

Publications

Treatment of rheumatoid arthritis using dual-targeted and dual-response intelligent micelles: a “three birds with one stone” strategy

Published: 2025-02-01

Journal: JOURNAL OF NANOBIOTECHNOLOGY

Application: IHC

Impact Factor: 12.60

Species: Rat

PMID: 39893385

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Free Secondary Antibody

Anti-MMP1 Antibody Picoband®

MMP1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Treatment of rheumatoid arthritis using dual-targeted and dual-response intelligent micelles: a “three birds with one stone” strategy

The expression and role of SUZ12 in lung adenocarcinoma

Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice

Electroacupuncture Prevents Osteoarthritis of High-Fat Diet-Induced Obese Rats

Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats

Bone marrow mesenchymal stem cells attenuate the progression of focal segmental glomerulosclerosis in rat models

BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation

Andrographolide plays an important role in bleomycin-induced pulmonary fibrosis treatment

Decreased expression of matrix metalloproteinase-1 in the maternal umbilical serum, trophoblasts and decidua leads to preeclampsia

Alterations of collagen-I, MMP-1 and TIMP-1 in the periodontal ligament of diabetic rats under mechanical stress

The Protective Effects of Long-Term Oral Administration of Marine Collagen Hydrolysate from Chum Salmon on Collagen Matrix Homeostasis in the Chronological Aged Skin of Sprague-Dawley Male Rats

Effects of exogenous urotensin II on vascular remodelling after balloon injury

IL-17 Stimulates Migration of Carotid Artery Vascular Smooth Muscle Cells in an MMP-9 Dependent Manner via p38 MAPK and ERK1/2-Dependent NF-κB and AP-1 Activation

Luteolin inhibits proliferation and affects the function of stimulated rat synovial fibroblasts

The adaptive response of periodontal ligament to orthodontic force loading – A combined biomechanical and biological study

Therapeutic effects of α-lipoic acid on bleomycin-induced pulmonary fibrosis in rats

Effect of Danshao Huaxian capsule on expression of matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in fibrotic liver of rats

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