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Product Info Summary
| SKU: | M30946-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone#RM219
SKU/Catalog Number
M30946-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone#RM219 (Catalog # M30946-2). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone#RM219 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30946-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM219
Isotype
Rabbit IgG
Immunogen
Mouse IgG2a
Cross-reactivity
This antibody reacts to the Fc region of mouse IgG2a. No cross-reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Reactive Species
M30946-2 is reactive to Ighg in Mouse
Calculated molecular weight
36.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30946-2 is guaranteed for ELISA, WB Boster Guarantee
Recommend Dilution
ELISA: 0.005 ug/mL – 0.2 ug/mL
WB: 0.5 ug/mL-2 ug/mL.
Validation Images & Assay Conditions
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Western blot of nonreduced(-) and reduced(+) mouse IgG2a, using 0.5ug/mL of clone RM219. This antibody reacts to nonreduced IgG2a (~150 kDa).
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ELISA of mouse immunoglobulins shows RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone#RM219 (M30946-2)
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1 Customer Q&As for Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone#RM219
Question
We are currently using anti-Mouse IgG2a Rabbit Monoclonal antibody, Clone#RM219 M30946-2 for mouse tissue, and we are content with the IP results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-16
Answer
The anti-Mouse IgG2a Rabbit Monoclonal antibody, Clone#RM219 (M30946-2) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-16
