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Product Info Summary
| SKU: | M33092 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108
SKU/Catalog Number
M33092
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108 (Catalog # M33092). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M33092)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM108
Isotype
Rabbit IgG
Immunogen
Mouse IgG2b
Cross-reactivity
This antibody reacts to the Fc region of mouse IgG2b. No cross-reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
Reactive Species
M33092 is reactive to Igh-3 in Mouse
Calculated molecular weight
44.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M33092 is guaranteed for ELISA, WB Boster Guarantee
Recommend Dilution
ELISA: 0.005 ug/mL – 0.2 ug/mL
WB: 0.1 ug/mL-0.5 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/mL of clone RM108. This antibody reacted to nonreduced IgG2b (~150 kDa) much stronger than the reduced Gamma 2b(~50 kDa).
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ELISA result showing specificity
ELISA of mouse immunoglobulins shows RM108 reacted to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108 (M33092)
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Customer Q&As
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1 Customer Q&As for Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108
Question
We are currently using anti-Mouse IgG2b Rabbit Monoclonal antibody, Clone#RM108 M33092 for mouse tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on dog tissues as well?
R. Banerjee
Verified customer
Asked: 2014-10-28
Answer
The anti-Mouse IgG2b Rabbit Monoclonal antibody, Clone#RM108 (M33092) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-28
