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Product Info Summary
| SKU: | M33092 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108
SKU/Catalog Number
M33092
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108 (Catalog # M33092). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M33092)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM108
Isotype
Rabbit IgG
Immunogen
Mouse IgG2b
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the Fc region of mouse IgG2b. No cross-reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
Reactive Species
M33092 is reactive to Igh-3 in Mouse
Applications
M33092 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
44.259kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 0.005 ug/mL – 0.2 ug/mL
WB: 0.1 ug/mL-0.5 ug/mL.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/mL of clone RM108. This antibody reacted to nonreduced IgG2b (~150 kDa) much stronger than the reduced Gamma 2b(~50 kDa).
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Figure 2. ELISA result showing specificity
ELISA of mouse immunoglobulins shows RM108 reacted to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Figure 3. ELISA result showing specificity
A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For Igh-3 (Source: Uniprot.org, NCBI)
Gene Name
Igh-3
Full Name
Ig gamma-2B chain C region
Weight
44.259kDa
Alternative Names
Ig gamma-2B chain C region
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Igh-3, check out the Igh-3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Igh-3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108 (M33092)
Hello CJ!
M33092 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Characterization of bone marrow-derived mesenchymal stem cells from dimethyloxallyl glycine-preconditioned mice: Evaluation of the feasibility of dimethyloxallyl glycine as a mobilization agent
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Mouse IgG2b Rabbit Monoclonal Antibody, Clone#RM108
Question
We are currently using anti-Mouse IgG2b Rabbit Monoclonal antibody, Clone#RM108 M33092 for mouse tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on dog tissues as well?
R. Banerjee
Verified customer
Asked: 2014-10-28
Answer
The anti-Mouse IgG2b Rabbit Monoclonal antibody, Clone#RM108 (M33092) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-28
