Anti-Interleukin-10 IL10 Antibody

IL-10 antibody

Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1015. Tested in ELISA, WB applications. This antibody reacts with Mouse. Cited in 10 publication(s).

Product Info Summary

SKU: RP1015
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Interleukin-10 IL10 Antibody

View all IL-10 Antibodies

SKU/Catalog Number

RP1015

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1015. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-10 IL10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1015)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse IL-10 recombinant protein (Position: S19-S178).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1015 is reactive to IL10 in Mouse

Applications

RP1015 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

20.517kDa

Background of IL-10

Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For IL10 (Source: Uniprot.org, NCBI)

Gene Name

IL10

Full Name

Interleukin-10

Weight

20.517kDa

Superfamily

IL-10 family

Alternative Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL10, check out the IL10 Infographic

IL10 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

RP1015 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Prevention and Alleviation of Dextran Sulfate Sodium Salt-Induced Inflammatory Bowel Disease in Mice With Bacillus subtilis-Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora

Chronic intestinal inflammation suppresses brain activity by inducing neuroinflammation in mice

The role of regulatory B cells in Echinococcus granulosus -infected mice

Hydrogen sulfide protects blood%u2013brain barrier integrity following cerebral ischemia

Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface

Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats

Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice

Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A

Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy

Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model

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15 Customer Q&As for Anti-Interleukin-10 IL10 Antibody

Question

Does RP1015 anti-IL10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

It shows on the product datasheet, RP1015 anti-IL10 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-20

Question

My colleagues were satisfied with the WB result of your anti-IL10 antibody. However we have been able to see positive staining in t-cell secreted. using this antibody. Is that expected? Could you tell me where is IL10 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

According to literature, t-cell does express IL10. Generally IL10 expresses in secreted. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510

Boster Scientific Support

Answered: 2019-11-18

Question

I was wanting to use your anti-IL10 antibody for ELISA for mouse esophagus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse esophagus identification?

C. Patel

Verified customer

Asked: 2019-11-13

Answer

It shows on the product datasheet, RP1015 anti-IL10 antibody has been validated for ELISA, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse esophagus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-13

Question

Does anti-IL10 antibody RP1015 work for ELISA with esophagus?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

According to the expression profile of esophagus, IL10 is highly expressed in esophagus. So, it is likely that anti-IL10 antibody RP1015 will work for ELISA with esophagus.

Boster Scientific Support

Answered: 2019-10-09

Question

We have been able to see staining in mouse esophagus. Are there any suggestions? Is anti-IL10 antibody supposed to stain esophagus positively?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

Based on literature esophagus does express IL10. Based on Uniprot.org, IL10 is expressed in esophagus, t-cell, among other tissues. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510

Boster Scientific Support

Answered: 2019-07-08

Question

My question regards to test anti-IL10 antibody RP1015 on mouse esophagus for research purposes, then I may be interested in using anti-IL10 antibody RP1015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

The products we sell, including anti-IL10 antibody RP1015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-04

Question

Is a blocking peptide available for product anti-IL10 antibody (RP1015)?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

We do provide the blocking peptide for product anti-IL10 antibody (RP1015). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-26

Question

Is this RP1015 anti-IL10 antibody reactive to the isotypes of IL10?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

The immunogen of RP1015 anti-IL10 antibody is E. coli-derived mouse IL-10 recombinant protein(Position: S19-S178). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-13

Question

We are currently using anti-IL10 antibody RP1015 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on primate tissues as well?

Z. Mitchell

Verified customer

Asked: 2018-10-18

Answer

The anti-IL10 antibody (RP1015) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-18

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody RP1015. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-01-12

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-12

Question

See attached the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody RP1015. Please let me know if you require anything else.

A. Brown

Verified customer

Asked: 2017-05-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-02

Question

We have tried in the past anti-IL10 antibody for ELISA on t-cell a few months ago. I am using mouse, and I plan to use the antibody for WB next. We are interested in examining t-cell as well as esophagus in our next experiment. Could give a recommendation on which antibody would work the best for WB?

J. Carter

Verified customer

Asked: 2016-03-10

Answer

I have checked the website and datasheets of our anti-IL10 antibody and it appears that RP1015 has been validated on mouse in both ELISA and WB. Thus RP1015 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2016-03-10

Question

Is there a BSA free version of anti-IL10 antibody RP1015 available?

R. Edwards

Verified customer

Asked: 2015-03-19

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL10 antibody RP1015 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-03-19

Question

I see that the anti-IL10 antibody RP1015 works with ELISA, what is the protocol used to produce the result images on the product page?

H. Parker

Verified customer

Asked: 2014-04-22

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-04-22

Question

I have a question about product RP1015, anti-IL10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Li

Verified customer

Asked: 2013-06-24

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1015 anti-IL10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-06-24

Order DetailsPrice
RP1015

100μg

$370
RP1015-10ug

10μg sample (liquid)

$99
RP1015-Biotin

100 μg Biotin conjugated

$570
RP1015-Cy3

100 μg Cy3 conjugated

$570
RP1015-Dylight488

100 μg Dylight488 conjugated

$570
RP1015-Dylight550

100 μg Dylight550 conjugated

$570
RP1015-Dylight594

100 μg Dylight594 conjugated

$570
RP1015-FITC

100 μg FITC conjugated

$570
RP1015-HRP

100 μg HRP conjugated

$570
RP1015-APC

100 μg APC conjugated

$670
RP1015-PE

100 μg PE conjugated

$670
RP1015-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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