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Product Info Summary
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Anti-Interleukin-10 IL10 Antibody
Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1015. Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-10 IL10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1015)
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
E. coli-derived mouse IL-10 recombinant protein (Position: S19-S178).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
RP1015 is reactive to Il10 in Mouse
RP1015 is guaranteed for ELISA, WB Boster Guarantee
Background of IL-10
Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Anti-mouse IL-10 antibody, RP1015, Western blotting
Lane 1: Recombinant Mouse IL-10 Protein 10ng
Lane 2: Recombinant Mouse IL-10 Protein 5ng
Lane 3: Recombinant Mouse IL-10 Protein 2
Gene/Protein Information For Il10 (Source: Uniprot.Org, NCBI)
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF Il10|CSIF, IL-, If2a, Il-10|interleukin 10|interleukin-10|cytokine synthesis inhibitory factor|interferon 2a*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il10, check out the Il10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
RP1015 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hydrogen sulfide protects blood%u2013brain barrier integrity following cerebral ischemia
Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface
Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats
Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice
Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A
Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy
Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model
Wang R, Lu M, Zhang J, Chen S, Luo X, Qin Y, Chen H. J Exp Clin Cancer Res. 2011 May 20;30:62. Doi: 10.1186/1756-9966-30-62. Increased Il-10 Mrna Expression In Tumor-Associated Macrophage Correlated With Late Stage Of Lung Cancer.
Zhan X, Jia L, Niu Y, Qi H, Chen X, Zhang Q, Zhang J, Wang Y, Dong L, Wang C. Biomaterials. 2014 Dec;35(38):10046-57. Doi: 10.1016/J.Biomaterials.2014.09.007. Epub 2014 Sep 22. Targeted Depletion Of Tumour-Associated Macrophages By An Alendronate-...
Jin Hb, Yang Yb, Song Yl, Zhang Yc, Li Yr. Mol Biol Rep. 2012 Dec;39(12):11005-9. Doi: 10.1007/S11033-012-2002-4. Epub 2012 Oct 8. Protective Roles Of Quercetin In Acute Myocardial Ischemia And Reperfusion Injury In Rats.
Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.
Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...
Huang Z, Zhang Z, Jiang Y, Zhang D, Chen J, Dong L, Zhang J. J Control Release. 2012 Mar 10;158(2):286-92. Doi: 10.1016/J.Jconrel.2011.11.013. Epub 2011 Nov 18. Targeted Delivery Of Oligonucleotides Into Tumor-Associated Macrophages For Cancer Imm...
Huang Z, Yang Y, Jiang Y, Shao J, Sun X, Chen J, Dong L, Zhang J. Biomaterials. 2013 Jan;34(3):746-55. Doi: 10.1016/J.Biomaterials.2012.09.062. Epub 2012 Oct 27. Anti-Tumor Immune Responses Of Tumor-Associated Macrophages Via Toll-Like Receptor 4 ...
Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.
Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...
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