Anti-Interleukin-10 IL10 Antibody

Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1015. Tested in ELISA, WB applications. This antibody reacts with Mouse. Cited in 16 publication(s).

Product Info Summary

SKU: RP1015
Size: 100μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Interleukin-10 IL10 Antibody

See all IL-10 products

SKU/Catalog Number

RP1015

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1015. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-10 IL10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1015)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived mouse IL-10 recombinant protein (Position: S19-S178).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1015 is reactive to Il10 in Mouse

Applications

RP1015 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Il10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P18893

Gene ID

16153

Gene Name

Il10

Full Name

Interleukin-10

Weight

20.641kDa

Superfamily

IL-10 family

Alternative Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF Il10|CSIF, IL-, If2a, Il-10|interleukin 10|interleukin-10|cytokine synthesis inhibitory factor|interferon 2a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il10, check out the Il10 Infographic

Il10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1015 has been cited in 16 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Hydrogen sulfide protects blood%u2013brain barrier integrity following cerebral ischemia

Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface

Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats

Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice

Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A

Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy

Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model

Wang R, Lu M, Zhang J, Chen S, Luo X, Qin Y, Chen H. J Exp Clin Cancer Res. 2011 May 20;30:62. Doi: 10.1186/1756-9966-30-62. Increased Il-10 Mrna Expression In Tumor-Associated Macrophage Correlated With Late Stage Of Lung Cancer.

Zhan X, Jia L, Niu Y, Qi H, Chen X, Zhang Q, Zhang J, Wang Y, Dong L, Wang C. Biomaterials. 2014 Dec;35(38):10046-57. Doi: 10.1016/J.Biomaterials.2014.09.007. Epub 2014 Sep 22. Targeted Depletion Of Tumour-Associated Macrophages By An Alendronate-...

Jin Hb, Yang Yb, Song Yl, Zhang Yc, Li Yr. Mol Biol Rep. 2012 Dec;39(12):11005-9. Doi: 10.1007/S11033-012-2002-4. Epub 2012 Oct 8. Protective Roles Of Quercetin In Acute Myocardial Ischemia And Reperfusion Injury In Rats.

Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.

Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...

Huang Z, Zhang Z, Jiang Y, Zhang D, Chen J, Dong L, Zhang J. J Control Release. 2012 Mar 10;158(2):286-92. Doi: 10.1016/J.Jconrel.2011.11.013. Epub 2011 Nov 18. Targeted Delivery Of Oligonucleotides Into Tumor-Associated Macrophages For Cancer Imm...

Huang Z, Yang Y, Jiang Y, Shao J, Sun X, Chen J, Dong L, Zhang J. Biomaterials. 2013 Jan;34(3):746-55. Doi: 10.1016/J.Biomaterials.2012.09.062. Epub 2012 Oct 27. Anti-Tumor Immune Responses Of Tumor-Associated Macrophages Via Toll-Like Receptor 4 ...

Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.

Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...

Have you used Anti-Interleukin-10 IL10 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Interleukin-10 IL10 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

15 Customer Q&As for Anti-Interleukin-10 IL10 Antibody

Question

Does RP1015 anti-IL10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

It shows on the product datasheet, RP1015 anti-IL10 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-20

Question

My colleagues were satisfied with the WB result of your anti-IL10 antibody. However we have been able to see positive staining in t-cell secreted. using this antibody. Is that expected? Could you tell me where is IL10 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

According to literature, t-cell does express IL10. Generally IL10 expresses in secreted. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510

Boster Scientific Support

Answered: 2019-11-18

Question

I was wanting to use your anti-IL10 antibody for ELISA for mouse esophagus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse esophagus identification?

C. Patel

Verified customer

Asked: 2019-11-13

Answer

It shows on the product datasheet, RP1015 anti-IL10 antibody has been validated for ELISA, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse esophagus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-13

Question

Does anti-IL10 antibody RP1015 work for ELISA with esophagus?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

According to the expression profile of esophagus, IL10 is highly expressed in esophagus. So, it is likely that anti-IL10 antibody RP1015 will work for ELISA with esophagus.

Boster Scientific Support

Answered: 2019-10-09

Question

We have been able to see staining in mouse esophagus. Are there any suggestions? Is anti-IL10 antibody supposed to stain esophagus positively?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

Based on literature esophagus does express IL10. Based on Uniprot.org, IL10 is expressed in esophagus, t-cell, among other tissues. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510

Boster Scientific Support

Answered: 2019-07-08

Question

My question regards to test anti-IL10 antibody RP1015 on mouse esophagus for research purposes, then I may be interested in using anti-IL10 antibody RP1015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

The products we sell, including anti-IL10 antibody RP1015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-04

Question

Is a blocking peptide available for product anti-IL10 antibody (RP1015)?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

We do provide the blocking peptide for product anti-IL10 antibody (RP1015). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-26

Question

Is this RP1015 anti-IL10 antibody reactive to the isotypes of IL10?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

The immunogen of RP1015 anti-IL10 antibody is E. coli-derived mouse IL-10 recombinant protein(Position: S19-S178). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-13

Question

We are currently using anti-IL10 antibody RP1015 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on primate tissues as well?

Z. Mitchell

Verified customer

Asked: 2018-10-18

Answer

The anti-IL10 antibody (RP1015) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-18

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody RP1015. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-01-12

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-12

Question

See attached the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody RP1015. Please let me know if you require anything else.

A. Brown

Verified customer

Asked: 2017-05-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-02

Question

We have tried in the past anti-IL10 antibody for ELISA on t-cell a few months ago. I am using mouse, and I plan to use the antibody for WB next. We are interested in examining t-cell as well as esophagus in our next experiment. Could give a recommendation on which antibody would work the best for WB?

J. Carter

Verified customer

Asked: 2016-03-10

Answer

I have checked the website and datasheets of our anti-IL10 antibody and it appears that RP1015 has been validated on mouse in both ELISA and WB. Thus RP1015 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2016-03-10

Question

Is there a BSA free version of anti-IL10 antibody RP1015 available?

R. Edwards

Verified customer

Asked: 2015-03-19

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL10 antibody RP1015 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-03-19

Question

I see that the anti-IL10 antibody RP1015 works with ELISA, what is the protocol used to produce the result images on the product page?

H. Parker

Verified customer

Asked: 2014-04-22

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2014-04-22

Question

I have a question about product RP1015, anti-IL10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Li

Verified customer

Asked: 2013-06-24

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1015 anti-IL10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-06-24

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
RP1015
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.