Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine™

Mouse CD169/SIGLEC-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Siglec1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1305
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine™

See all Siglec-1/CD169 products

SKU/Catalog Number

EK1305

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CD169/SIGLEC-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Siglec1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1305)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T20-R1639

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1305 is reactive to Siglec1 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1305 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1305-CAP Anti-Mouse Siglec1 Pre-coated 96-well strip microplate 1
EK1305-ST Mouse Siglec1 Standard 2 vials, 10 ng/tube
EK1305-DA Mouse Siglec1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0110.1860.3340.5440.9731.5712.0312.394

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Siglec-1/CD169, Dilution ratio of 1:10, concentration in serum is 7-16ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3311301554035112585216
Standard deviation21.1884.56371.1826.6786.8432.92
CV (%)6.4%6.5%6.7%7.6%6.9%8.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Siglec-1/CD169 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 133136734835134912.773.6%
Sample 21301126012141306127037.052.9%
Sample 355405164513548865181233.614.5%
*number of samples for each test n=16.

Gene/Protein Information For Siglec1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q62230

Gene ID

20612

Gene Name

Siglec1

Full Name

Sialoadhesin

Weight

182.979kDa

Superfamily

immunoglobulin superfamily

Alternative Names

CD169; FLJ00051; sialic acid binding Ig-like lectin 1, sialoadhesin; sialoadhesin; Siglec1; Siglec-1 Siglec1|Cd169, Sigle, Siglec-1, Sn|sialic acid binding Ig-like lectin 1, sialoadhesin|sialoadhesin|SER|sheep erythrocyte receptor|sialic acid-binding Ig-like lectin 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Siglec1, check out the Siglec1 Infographic

Siglec1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Siglec1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1305

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

5 Customer Q&As for Mouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine™

Question

Q: is it okay to use citrate plasma as samples in Mouse CD169 Picokine® ELISA Kit (Catalog # EK1305)?

Verified Customer

Verified customer

Asked: 2020-05-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CD169 causing degradation of its protein structure. CD169 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD169 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-05-22

Question

Q: which procedure should I follow in order to thaw whole blood sample for CD169 ELISA after freezing?

Y. Bhatt

Verified customer

Asked: 2020-05-07

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD169 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-05-07

Question

Q: What is the optimal O.D. value for CD169 ELISA kit? I used your CD169 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD169 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD169 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD169 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-01-29

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-11-09

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-11-09

Question

Q: if the enzyme conjugated CD169 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD169 antigen?

W. Martin

Verified customer

Asked: 2019-10-09

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-10-09

$369

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK1305
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.