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Product Info Summary
| SKU: | A14080-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MRP-S25 Antibody
SKU/Catalog Number
A14080-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRP-S25 Antibody catalog # A14080-1. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-S25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14080-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS25. AA range:124-173
Reactive Species
A14080-1 is reactive to MRPS25 in Human, Mouse, Rat
Calculated molecular weight
20.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14080-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of various cells using MRP-S25 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from 293 cells, using MRPS25 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using MRPS25 antibody.
Specific Publications For Anti-MRP-S25 Antibody (A14080-1)
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Customer Q&As
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5 Customer Q&As for Anti-MRP-S25 Antibody
Question
Does A14080-1 anti-MRP-S25 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
You can see on the product datasheet, A14080-1 anti-MRP-S25 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-03
Question
Is this A14080-1 anti-MRP-S25 antibody reactive to the isotypes of MRPS25?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The immunogen of A14080-1 anti-MRP-S25 antibody is Synthesized peptide derived from the C-terminal region of human MRP-S25. at AA rangle: 110-190. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-09
Question
We are currently using anti-MRP-S25 antibody A14080-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
The anti-MRP-S25 antibody (A14080-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-05
Question
I have a question about product A14080-1, anti-MRP-S25 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14080-1 anti-MRP-S25 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-25
Question
I am looking for to test anti-MRP-S25 antibody A14080-1 on rat left lobe of thyroid gland for research purposes, then I may be interested in using anti-MRP-S25 antibody A14080-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Anderson
Verified customer
Asked: 2017-11-20
Answer
The products we sell, including anti-MRP-S25 antibody A14080-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-20
