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Product Info Summary
| SKU: | A13823 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-MRP-S35 Antibody
SKU/Catalog Number
A13823
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRP-S35 Antibody catalog # A13823. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRP-S35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13823)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS35. AA range:211-260
Reactive Species
A13823 is reactive to MRPS35 in Human, Mouse
Calculated molecular weight
36.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13823 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of K562 cells using MRP-S35 Polyclonal Antibody
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Western blot analysis of lysates from Jurkat, COLO, and A549 cells, using MRPS35 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Immunofluorescence analysis of MCF7 cell. 1, primary Antibody was diluted at 1:100 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody was diluted at 1:500 (room temperature, 50min).
Specific Publications For Anti-MRP-S35 Antibody (A13823)
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Customer Q&As
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5 Customer Q&As for Anti-MRP-S35 Antibody
Question
I see that the anti-MRP-S35 antibody A13823 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-23
Question
I was wanting to use your anti-MRP-S35 antibody for WB for human embl:aah63515.1} rc pancreas eco:0000312 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human embl:aah63515.1} rc pancreas {eco:0000312 identification?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
It shows on the product datasheet, A13823 anti-MRP-S35 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human embl:aah63515.1} rc pancreas {eco:0000312 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-31
Question
Will anti-MRP-S35 antibody A13823 work for WB with embl:aah63515.1} rc pancreas {eco:0000312?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
According to the expression profile of embl:aah63515.1} rc pancreas eco:0000312, MRPS35 is highly expressed in embl:aah63515.1 rc pancreas eco:0000312. So, it is likely that anti-MRP-S35 antibody A13823 will work for WB with embl:aah63515.1 rc pancreas {eco:0000312.
Boster Scientific Support
Answered: 2019-12-10
Question
I have a question about product A13823, anti-MRP-S35 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13823 anti-MRP-S35 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-22
Question
We are currently using anti-MRP-S35 antibody A13823 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
V. Li
Verified customer
Asked: 2017-10-06
Answer
The anti-MRP-S35 antibody (A13823) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-06
