Human SCF/KITLG/Kit ligand ELISA Kit PicoKine®

SCF/c-kit Ligand ELISA kit for Human

Human SCF ELISA Kit PicoKine™ (96 Tests). Quantitate Human KITLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0507
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human SCF/KITLG/Kit ligand ELISA Kit PicoKine®

View all SCF/c-kit Ligand ELISA kits

SKU/Catalog Number

EK0507

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human SCF ELISA Kit PicoKine™ (96 Tests). Quantitate Human KITLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human SCF/KITLG/Kit ligand ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0507)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E26-A190

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0507 for more details

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

EK0507 is reactive to KITLG in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0507 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of SCF/c-kit Ligand

Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.

Kit Components

Catalog Number Description Quantity
EK0507-CAP Anti-Human KITLG Pre-coated 96-well strip microplate 1
EK0507-ST Human KITLG Standard 2 vials, 10 ng/tube
EK0507-DA Human KITLG Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.2562.512525050010002000
O.D.0.0040.1020.1750.3330.5591.0211.6722.054

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect SCF/c-kit Ligand, Dilution ratio of 1:1, concentration in serum and plasma is 446-1729 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human SCF/KITLG/Kit ligand ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)9227486687296875
Standard deviation7.1715.7%147.637.3917.1664.75
CV (%)7.8%5.7%5.5%8.5%5.8%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of SCF/c-kit Ligand in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 192949796941.922%
Sample 22742742892832806.362.2%
Sample 386687494786988933.63.7%
*number of samples for each test n=16.

Gene/Protein Information For KITLG (Source: Uniprot.Org, NCBI)

Gene Name

KITLG

Full Name

Kit ligand

Weight

30.899kDa

Superfamily

SCF family

Alternative Names

c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor KITLG DCUA, DFNA69, FPH2, FPHH, KL-1, Kitl, MGF, SCF, SF, SHEP7, SLF KIT ligand kit ligand|c-Kit ligand|familial progressive hyperpigmentation 2|mast cell growth factor|steel factor|stem cell factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on KITLG, check out the KITLG Infographic

KITLG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for KITLG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0507 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

GM-CSF Inhibits c-Kit and SCF Expression by Bone Marrow-Derived Dendritic Cells

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6 Customer Q&As for Human SCF/KITLG/Kit ligand ELISA Kit PicoKine®

Question

Q: Can SCF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-11-19

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification SCF using the EK0507 Human SCF Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-09-30

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2018-09-30

Question

Q: is it okay to use heparin plasma as samples in Human SCF Picokine® ELISA Kit (Catalog # EK0507)?

R. Thompson

Verified customer

Asked: 2018-08-20

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of SCF causing degradation of its protein structure. SCF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the SCF ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-08-20

Question

Q: What is the optimal O.D. value for SCF ELISA kit? I performed your SCF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain SCF even though the O.D. values are not very high?

B. Gandhi

Verified customer

Asked: 2018-06-19

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SCF ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of SCF that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-06-19

Question

Q: how do I analyze ELISA data? I have obtained SCF level in serum.

Verified Customer

Verified customer

Asked: 2018-01-12

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-01-12

Question

Q: can you suggest the dilution ratio of serum samples for detection of SCF in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2017-08-06

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SCF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2017-08-06

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