Click image to see more details
Product Info Summary
| SKU: | M00196-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Dog, Mouse |
| Host: | Hamster |
| Application: | Flow Cytometry, IP, IHC-F |
Product info
Product Name
Anti-Ms CD80 Purified Monoclonal Antibody
SKU/Catalog Number
M00196-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ms CD80 Purified Monoclonal Antibody (Catalog# M00196-2). Tested in Flow Cytometry, IP, IHC-F application(s). This antibody reacts with Mouse, Dog.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ms CD80 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00196-2)
Host
Hamster
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
16-10A1
Isotype
Armenian Hamster IgG2
Immunogen
CD80-transfected CHO cell line. The Armenian hamster monoclonal antibody 16-10A1 reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Reactive Species
M00196-2 is reactive to CD80 in Dog, Mouse
Calculated molecular weight
34.6 kDa
Background of CD80
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00196-2 is guaranteed for Flow Cytometry, IP, IHC-F Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
Click image to see more details
Separation of murine CD80 positive monocytes (red-filled) from CD80 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine peritoneal fluid cells stained using anti-mouse CD80 (16-10A1) purified antibody (concentration in sample 2 µg/ml) GAM APC.
Click image to see more details
Flow cytometry surface staining pattern of murine peripheral whole blood stained using anti-mouse CD80 (16-10A1) purified antibody (concentration in sample 2 µg/ml) GAM APC.
Specific Publications For Anti-Ms CD80 Purified Monoclonal Antibody (M00196-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Ms CD80 Purified Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Ms CD80 Purified Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
