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- Table of Contents
CD80 antibody (A00196) performs well in ELISA, IF, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Immunofluorescence Validation of CD80 in Human Tonsil
Immunofluorescent analysis of 4% paraformaldehyde-fixed human tonsil tissue labeling CD80 with A00196 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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Anti-CD80 Antibody
CD80 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1-4 μg/mL; IHC-P/IF: 5-20 μg/mL.
Antibody validated: Western Blot in human
mouse and rat samples; Immunofluorescence in the human
mouse and rat samples. All other applications and species not yet tested.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-CD80 Antibody
View all CD80 Antibodies
View all CD80 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
CD80 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
CD80 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CD80 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human CD80. The immunogen is located within amino acids 60 - 110 of CD80.
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
33.0 kDa
Background of CD80
CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Cite This Product
Anti-CD80 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00196)
Publications
Application:
Species:
Chen X,Gao A,Zhang F,Yang Z,Wang S,Fang Y,Li J,Wang J,Shi W,Wang L, Zheng Y,Sun Y.ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation.Theranostics.
Species: Human,Mouse
PMID: 33537094
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Product Promise
We promise Anti-CD80 Antibody performs excellently in relevant applications for ELISA, IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00196
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026