Click image to see more details
Product Info Summary
| SKU: | PA1365-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD80 Antibody Picoband®
SKU/Catalog Number
PA1365-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD80 Antibody catalog # PA1365-1. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD80 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1365-1)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80, different from the related mouse sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1365-1 is reactive to CD80 in Rat
Observed Molecular Weight
50 kDa
Background of CD80
Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al. (1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1365-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Positive Control
WB: Rat Thymus Tissue
Validation Images & Assay Conditions
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Anti-CD80 antibody, PA1365-1, Western blotting
All lanes: Anti CD80 (PA1365-1) at 0.5ug/ml
WB: Rat Thymus Tissue Lysate at 50ug
Predicted bind size: 34KD
Observed bind size: 50KD
Specific Publications For Anti-CD80 Antibody Picoband® (PA1365-1)
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Customer Q&As
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15 Customer Q&As for Anti-CD80 Antibody Picoband®
Question
Will PA1365-1 anti-CD80 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
It shows on the product datasheet, PA1365-1 anti-CD80 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-22
Question
We are currently using anti-CD80 antibody PA1365-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The anti-CD80 antibody (PA1365-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-11
Question
Does anti-CD80 antibody PA1365-1 work for WB with gastrocnemius?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
According to the expression profile of gastrocnemius, CD80 is highly expressed in gastrocnemius. So, it is likely that anti-CD80 antibody PA1365-1 will work for WB with gastrocnemius.
Boster Scientific Support
Answered: 2020-02-21
Question
I see that the anti-CD80 antibody PA1365-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-23
Question
Is there a BSA free version of anti-CD80 antibody PA1365-1 available?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD80 antibody PA1365-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-24
Question
I am looking for to test anti-CD80 antibody PA1365-1 on rat gastrocnemius for research purposes, then I may be interested in using anti-CD80 antibody PA1365-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Miller
Verified customer
Asked: 2019-10-18
Answer
The products we sell, including anti-CD80 antibody PA1365-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-18
Question
Is this PA1365-1 anti-CD80 antibody reactive to the isotypes of CD80?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The immunogen of PA1365-1 anti-CD80 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80(227-245aa DAHVSQNFTWEKPPEDPPD), different from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-27
Question
I was wanting to use your anti-CD80 antibody for WB for rat gastrocnemius on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat gastrocnemius identification?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
You can see on the product datasheet, PA1365-1 anti-CD80 antibody has been tested for WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat gastrocnemius in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-06
Question
Can you help my question with product PA1365-1, anti-CD80 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Kulkarni
Verified customer
Asked: 2018-11-28
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1365-1 anti-CD80 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-28
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for gastrocnemius using anti-CD80 antibody PA1365-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-25
Question
Is a blocking peptide available for product anti-CD80 antibody (PA1365-1)?
Verified Customer
Verified customer
Asked: 2018-06-07
Answer
We do provide the blocking peptide for product anti-CD80 antibody (PA1365-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-06-07
Question
Here is the WB image, lot number and protocol we used for gastrocnemius using anti-CD80 antibody PA1365-1. Please let me know if you require anything else.
D. Patel
Verified customer
Asked: 2017-07-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-05
Question
We have been able to see staining in rat gastrocnemius. What should we do? Is anti-CD80 antibody supposed to stain gastrocnemius positively?
R. Collins
Verified customer
Asked: 2015-06-11
Answer
According to literature gastrocnemius does express CD80. According to Uniprot.org, CD80 is expressed in gastrocnemius, lymphoid tissue, brain, erythroleukemia, among other tissues. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173
Boster Scientific Support
Answered: 2015-06-11
Question
Our team were content with the WB result of your anti-CD80 antibody. However we have seen positive staining in lymphoid tissue membrane using this antibody. Is that expected? Could you tell me where is CD80 supposed to be expressed?
J. Johnson
Verified customer
Asked: 2014-01-06
Answer
Based on literature, lymphoid tissue does express CD80. Generally CD80 expresses in membrane. Regarding which tissues have CD80 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Lymphoid tissue, Pubmed ID: 2794510, 1377173
Boster Scientific Support
Answered: 2014-01-06
Question
Does anti-CD80 antibody PA1365-1 work on goat WB with lymphoid tissue?
M. Anderson
Verified customer
Asked: 2013-08-30
Answer
Our lab technicians have not validated anti-CD80 antibody PA1365-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CD80 antibody PA1365-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat lymphoid tissue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-08-30
