Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80(CD80) detection. Tested with WB in Rat. Cited in 1 publication(s).
Anti-CD80 Antibody Info At A Glance
|Product Name||Anti-CD80 Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for B7 1/CD80 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Rat. B7 1/CD80 information: Molecular Weight: 36298 MW; .|
|Cite This Product||Anti-CD80 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1365-1)|
|Similar Products From
|Anti-CD80 Antibody may replace the following items: sc 133821|sc 161130|sc 161129|sc 514049.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of rat CD80(227-245aa DAHVSQNFTWEKPPEDPPD), different from the related mouse sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CD80 (Source: Uniprot.org, NCBI)
|Molecular Weight||36298 MW|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CD80 (Source: Uniprot.org, NCBI)
|Background||Cluster of Differentiation 80(also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28(for autoregulation and intercellular association) and CTLA-4(for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al.(1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.|
Our Boster Quality Guarantee for Anti-CD80 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti CD80 (PA1365-1) at 0.5ug/ml
WB: Rat Thymus Tissue Lysate at 50ug
Predicted bind size: 34KD
Observed bind size: 50KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
|Du Hy, Dong Lh, Zhao Bj, Fu J, Wang Qq, Chen F, Ou L, Li N, Sun X, Tang Zm, Song Hf. Acta Pharmacol Sin. 2012 Aug;33(8):1047-54. Doi: 10.1038/Aps.2012.54. Epub 2012 Jun 25. Immunostimulatory And Anti-Neoplasm Effects Of A Novel Palindrome Cpg Olig...||PubMed ID 22728711|
Order Product (PA1365-1)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.