Rabbit IgG polyclonal antibody for T-lymphocyte activation antigen CD80(CD80) detection. Tested with WB in Human. Cited in 1 publication(s).
Anti-CD80 Antibody Info At A Glance
|Product Name||Anti-CD80 Antibody
See all B7-1/CD80 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for B7 1/CD80 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. B7 1/CD80 information: Molecular Weight: 33048 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein; Tissue Specificity: Expressed on activated B-cells, macrophages and dendritic cells.|
|Cite This Product||Anti-CD80 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1365)|
|Similar Products From
|Anti-CD80 Antibody may replace the following items: sc 11027|sc 11033|sc 11034|sc 271878|sc 11027|sc 11030|sc 11032|sc 11033|sc 11034|sc 25660|sc 271878.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human CD80(57-71aa EELAQTRIYWQKEKK).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CD80 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||941|
|Species Of This Entry||Human|
|Protein Name||T-lymphocyte activation antigen CD80|
|Alternative Names||B7-1/CD80|Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80|
|Post Tranlational Modifications||Phosphorylation; Glycosylation; Methylation; Cleavage; Acetylation; Reduction; Biotinylation; Ribosylation; Acylation; Myristoylation; Demethylation; Phosphorothioation; Prenylation; Ubiquitination; Nitration; Geranylgeranylation; Carboxylation; Dephosphorylation; Farnesylation; Palmitoylation|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Gene Location||3q13.33, on Chromasome 3, gene sequence: NC_000003.12 (119524293..119559614, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CD80 (Source: Uniprot.org, NCBI)
|Protein Function||Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.|
|Interacting Proteins||CD274; CTLA4; NGFR|
|Research Areas||Adaptive Immunity; Cytokine Research; Immunology; Signal Transduction|
|Related Diseases||Neoplasms; Malignant Neoplasms; Autoimmune Reaction; Inflammation; Tissue Adhesions; Autoimmune Diseases; Carcinoma; Leukemia; Decreased Immunologic Activity [pe]; Autoimmunity; Melanoma; Lymphoma; Malignant Paraganglionic Neoplasm; Infective Disorder; Arthritis; Hiv Infections; Immunologic Deficiency Syndromes; Neoplasm Metastasis; Myeloid Leukemia|
|Related Pathways||Immune Response; Cell Activation; Secretion; Cell Proliferation; T Cell Activation; Cytokine Production; T Cell Proliferation; Pathogenesis; Cell Differentiation; Hypersensitivity; Cell Maturation; Cytokine Secretion; Cell Death; Sensitization; Phagocytosis; Endocytosis; Localization; Lymphocyte Proliferation; Tolerance Induction; Cell Adhesion|
|Background||Cluster of Differentiation 80(also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28(for autoregulation and intercellular association) and CTLA-4(for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. The CD80 genes encode B7-1 which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. Reeves et al.(1997) stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.|
|Scientific References||PMID: 2794510 by Freeman G.J., et al. B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells.|
PMID: 1377173 by Selvakumar A., et al. Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7.
Our Boster Quality Guarantee for Anti-CD80 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Recombinant Human CD80 Protein 10ng
Lane 2: Recombinant Human CD80 Protein 5ng
Lane 3: Recombinant Human CD80 Protein 2.5ng
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 1
|Du Hy, Dong Lh, Zhao Bj, Fu J, Wang Qq, Chen F, Ou L, Li N, Sun X, Tang Zm, Song Hf. Acta Pharmacol Sin. 2012 Aug;33(8):1047-54. Doi: 10.1038/Aps.2012.54. Epub 2012 Jun 25. Immunostimulatory And Anti-Neoplasm Effects Of A Novel Palindrome Cpg Olig...||PubMed ID 22728711|
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Note: sample size takes longer to process, average 5-7 days.
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