Anti-Ms CD90.2 Purified Azide Free Thy1 Monoclonal Antibody

Product Name

Anti-Ms CD90.2 Purified Azide Free Thy1 Monoclonal Antibody

View all THY1.2 Antibodies

SKU/Catalog Number

M01818-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Ms CD90.2 Purified Azide Free Thy1 Monoclonal Antibody (Catalog# M01818-2). Tested in Flow Cytometry, WB, ICC, Functional Assay application(s). This antibody reacts with Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Ms CD90.2 Purified Azide Free Thy1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01818-2)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, azide-free

Clonality

Monoclonal

Clone Number

1aG4/C5

Isotype

Mouse IgG3

Immunogen

Thymocytes from C3H/Di mice (Thy-1.2 positive). The mouse monoclonal antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90). Thy-1 is an 18-35 kDa cell surface GPI anchored glycoprotein (immunoglobulin superfamily); in mouse it is abundant on thymocytes and peripheral T lymphocytes.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M01818-2 is reactive to THY1.2 in Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

18.1 kDa

Background of THY1.2

CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. In the mouse, CD90 is expressed mainly on thymocytes and peripheral T lymphocytes. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01818-2 is guaranteed for Flow Cytometry, ICC, WB, Functional Assay Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Functional application: The bound of antibody 1aG4/C5 to the transfected mast cells induces the activation processes which are similar, but not identical, to the antigen activation of IgE stimulated cells.
Immunoprecipitation: The antibody 1aG4/C5 precipitates complexes with Lyn protein tyrosine kinase.
Flow cytometry: 1 μg/ml.

Validation Images & Assay Conditions

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See full target information THY1.2

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