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Product Info Summary
| SKU: | A04112-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-MST-3 Antibody
SKU/Catalog Number
A04112-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MST-3 Antibody catalog # A04112-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MST-3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04112-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human STK24. AA range:319-368
Reactive Species
A04112-1 is reactive to STK24 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
49.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04112-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using STK24 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using MST-3 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from COLO cells, using STK24 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from COLO205 cells using STK24 antibody.
Specific Publications For Anti-MST-3 Antibody (A04112-1)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-MST-3 Antibody
Question
I have a question about product A04112-1, anti-MST-3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Mitchell
Verified customer
Asked: 2020-01-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04112-1 anti-MST-3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-23
Question
Does anti-MST-3 antibody A04112-1 work on feline WB with secondary oocyte?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Our lab technicians have not validated anti-MST-3 antibody A04112-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-MST-3 antibody A04112-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline secondary oocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-19
Question
Is this A04112-1 anti-MST-3 antibody reactive to the isotypes of STK24?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The immunogen of A04112-1 anti-MST-3 antibody is Synthesized peptide derived from the C-terminal region of human MST-3. at AA rangle: 290-370. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-14
Question
Does A04112-1 anti-MST-3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
As indicated on the product datasheet, A04112-1 anti-MST-3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-28
Question
I see that the anti-MST-3 antibody A04112-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-23
Question
Do you have a BSA free version of anti-MST-3 antibody A04112-1 available?
Verified Customer
Verified customer
Asked: 2018-10-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MST-3 antibody A04112-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-15
Question
We want to test anti-MST-3 antibody A04112-1 on mouse skin for research purposes, then I may be interested in using anti-MST-3 antibody A04112-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Edwards
Verified customer
Asked: 2017-11-27
Answer
The products we sell, including anti-MST-3 antibody A04112-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-27
Question
Is a blocking peptide available for product anti-MST-3 antibody (A04112-1)?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
We do provide the blocking peptide for product anti-MST-3 antibody (A04112-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-07-10
Question
I was wanting to use your anti-MST-3 antibody for WB for mouse skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse skin identification?
J. Jones
Verified customer
Asked: 2017-04-13
Answer
You can see on the product datasheet, A04112-1 anti-MST-3 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-MST-3 antibody A04112-1. Let me know if you need anything else.
D. Dhar
Verified customer
Asked: 2017-03-20
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-20
Question
See below the WB image, lot number and protocol we used for skin using anti-MST-3 antibody A04112-1. Please let me know if you require anything else.
L. Brown
Verified customer
Asked: 2016-03-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-17
Question
We are currently using anti-MST-3 antibody A04112-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
M. Edwards
Verified customer
Asked: 2015-09-30
Answer
The anti-MST-3 antibody (A04112-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-30
Question
Would anti-MST-3 antibody A04112-1 work for WB with skin?
M. Roberts
Verified customer
Asked: 2014-06-04
Answer
According to the expression profile of skin, STK24 is highly expressed in skin. So, it is likely that anti-MST-3 antibody A04112-1 will work for WB with skin.
Boster Scientific Support
Answered: 2014-06-04
