Anti-MTBP Monoclonal Antibody

Product Name

Anti-MTBP Monoclonal Antibody

View all MTBP Antibodies

SKU/Catalog Number

M08842

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-MTBP Monoclonal Antibody (Catalog # M08842). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-MTBP Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08842)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: PHL-1

Isotype

IgG1 kappa

Immunogen

This monoclonal antibody was produced by repeated immunizations with a recombinant amino-terminal HIS_6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M08842 is reactive to MTBP in Human

Observed Molecular Weight

47 kDa

Calculated molecular weight

102.2 kDa

Background of MTBP

The MDM2 onco-protein is over expressed in human cancer cells and it promotes tumorigenesis by directly blocking the function of the p53 tumor suppressor. Studies also indicate that MDM2 regulates cell proliferation via p53 independent mechanisms. MTBP (Mdm2 [Two] Binding Protein) was identified by a yeast two-hybrid screen as an MDM2 binding protein (1). MTBP has been reported to modulate cell cycle progression by a p53 independent mechanism and suppress MDM2-mediated p53 degradation when ectopically expressed in cells (1,2). The human MTBP gene localizes to 8q24 (3) and MTBP RNA is expressed in a wide range of tissues, with highest being in the thymus, testes and ovaries (1).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M08842 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:20,000
WB: 1:200 - 1:2,000
This antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 110 kDa in size corresponding to MTBP by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information MTBP

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