Anti-mtTFA TFAM Antibody Picoband™ (monoclonal, 4D9)

mtTFA antibody

Boster Bio Anti-mtTFA TFAM Antibody Picoband™ (monoclonal, 4D9) catalog # M01119-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M01119-1
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IHC, WB

Product Name

Anti-mtTFA TFAM Antibody Picoband™ (monoclonal, 4D9)

View all mtTFA Antibodies

SKU/Catalog Number

M01119-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-mtTFA TFAM Antibody Picoband™ (monoclonal, 4D9) catalog # M01119-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-mtTFA TFAM Antibody Picoband™ (monoclonal, 4D9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01119-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

4D9

Isotype

IgG2b

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA (214-241aa EMKSWEEQMIEVGRKDLLRRTIKKQRKY), different from the related mouse and rat sequences by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M01119-1 is reactive to TFAM in Human

Applications

M01119-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Background of mtTFA

TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 1-2μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For TFAM (Source: Uniprot.Org, NCBI)

Uniprot ID

Q00059

Gene ID

7019

Gene Name

TFAM

Full Name

Transcription factor A, mitochondrial

Weight

29.097kDa

Alternative Names

mitochondrial transcription factor A; MtTF1; mtTFA; TCF-6; TCF6L1; TCF6L2Mitochondrial transcription factor 1; TCF6TCF6L3; Transcription factor 6; Transcription factor 6-like 2 (mitochondrial transcription factor); Transcription factor 6-like 2; transcription factor A, mitochondrial TFAM MTDPS15, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3 transcription factor A, mitochondrial transcription factor A, mitochondrial|mitochondrial transcription factor 1|mitochondrial transcription factor A|transcription factor 6|transcription factor 6-like 1|transcription factor 6-like 2 (mitochondrial transcription factor)|transcription factor 6-like 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TFAM, check out the TFAM Infographic

TFAM infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TFAM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M01119-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Shen W,Jia N,Miao J,Chen S,Zhou S,Meng P,Zhou X,Tang L,Zhou L:Penicilliumin B Protects against Cisplatin-Induced Renal Tubular Cell Apoptosis through Activation of AMPK-Induced Autophagy and Mitochondrial Biogenesis.Kidney Dis 2021.doi:10.1159/000514657
Species: Human,Mouse
M01119-1 usage in article: APP:WB, SAMPLE:HKC-8 CELL, DILUTION:NA

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4 Customer Q&As for Anti-mtTFA TFAM Antibody Picoband™ (monoclonal, 4D9)

Question

We have been able to see staining in human lymphocyte. What should we do? Is anti-mtTFA antibody (monoclonal, 4D9) supposed to stain lymphocyte positively?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

From what I have seen in literature lymphocyte does express TFAM. From what I have seen in Uniprot.org, TFAM is expressed in female gonad, hepatoma, tongue, lymphocyte, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have TFAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymphocyte, Pubmed ID: 1610904
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-11-15

Question

My team were happy with the WB result of your anti-mtTFA antibody (monoclonal, 4D9). However we have been able to see positive staining in lymphocyte mitochondrion using this antibody. Is that expected? Could you tell me where is TFAM supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

Based on literature, lymphocyte does express TFAM. Generally TFAM expresses in mitochondrion. Regarding which tissues have TFAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymphocyte, Pubmed ID: 1610904
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-10-15

Question

Our lab used your anti-mtTFA antibody (monoclonal, 4D9) for Flow Cytometry on cervix carcinoma a few years ago. I am using human, and We intend to use the antibody for WB next. Our lab want to know about examining cervix carcinoma as well as female gonad in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

I looked at the website and datasheets of our anti-mtTFA antibody (monoclonal, 4D9) and I see that M01119-1 has been validated on human in both Flow Cytometry and WB. Thus M01119-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-05

Question

We are currently using anti-mtTFA antibody (monoclonal, 4D9) M01119-1 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

P. Jackson

Verified customer

Asked: 2013-02-07

Answer

The anti-mtTFA antibody (monoclonal, 4D9) (M01119-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-02-07

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:M01119-1

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M01119-1
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