Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody
Rabbit IgG polyclonal antibody for Muscarinic acetylcholine receptor M2(CHRM2) detection. Tested with WB, IHC-P in Human; Mouse; Rat.
|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody
See all CHRM2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for MACHR M2/CHRM2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MACHR M2/CHRM2 information: Molecular Weight: 51715 MW; Subcellular Localization: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Phosphorylation in response to agonist binding promotes receptor internalization.|
|Cite This Product||Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1325-1)|
|Specificity||Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody (PA1325-1) reacts with Human, Mouse, Rat CHRM2, in native form and recombinant. Superfamily members of CHRM2 are not reactive to PA1325-1.|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Muscarinic Acetylcholine Receptor 2(356-370aa EKQNIVARKIVKMTK), identical to the related rat and mouse sequences.|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody (PA1325-1).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-Muscarinic Acetylcholine Receptor 2 antibody, PA1325-1, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Muscarinic acetylcholine receptor M2|
|Alternative Names||Muscarinic acetylcholine receptor M2;CHRM2;|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Phosphorylation in response to agonist binding promotes receptor internalization. .|
|Molecular Weight||51715 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol. .|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||The muscarinic acetylcholine receptor M2, also known as the cholinergic receptor, muscarinic 2, is a muscarinic acetylcholine receptor that in humans is encoded by the CHRM2 gene. The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene.|
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We promise all of our products perform as described in datasheets.
Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to achr antibody, muscarinic acetylcholine receptor m2 antibody