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MYBBP1A antibody (A04187-2) performs well in ELISA, IF, ICC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of MYBBP1A using anti-MYBBP1A antibody (A04187-2) and anti-Beta Tubulin antibody (M01857-3).
MYBBP1A was detected in an immunocytochemical section of NIH/3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MYBBP1A Antibody (A04187-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
MYBBP1A was detected in an immunocytochemical section of NIH/3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MYBBP1A Antibody (A04187-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Designing a Western blot for MYBBP1A? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the MYBBP1A Western Blot GuideBundle Save
Anti-MYBBP1A Antibody Picoband®
MYBBP1A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IF, ICC, WB |
Tested Applications
MYBBP1A antibody was positively detected by WB in:
rat RH35 whole cell, mouse ovary tissue, mouse NIH/3T3 tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MYBBP1A antibody was positively detected by ICC/IF in:
NIH/3T3 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Mouse
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-MYBBP1A Antibody Picoband®
View all MYBBP1A Antibodies
View all MYBBP1A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived mouse MYBBP1A recombinant protein (Position: D39-R1316). Mouse MYBBP1A shares 69.7% and 89% amino acid (aa) sequence identity with human and rat MYBBP1A, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
150 kDa
Calculated Molecular Weight
152.0 kDa
Background of MYBBP1A
Enables E-box binding activity and transcription corepressor activity. Involved in circadian regulation of gene expression and negative regulation of DNA-templated transcription. Acts upstream of or within respiratory electron transport chain. Located in cytoplasm and nucleolus. Part of NLS-dependent protein nuclear import complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; integumental system; and limb. Orthologous to human MYBBP1A (MYB binding protein 1a).
Cite This Product
Anti-MYBBP1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04187-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
89%
Moderate Homology
Human
70%
Moderate Homology
Cow
69%
Low homology
Monkey
69%
Low homology
Sheep
69%
Low homology
Dog
68%
Low homology
Zebrafish
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for MYBBP1A antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MYBBP1A antibody
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Product Promise
We promise Anti-MYBBP1A Antibody Picoband® performs excellently in relevant applications for ELISA, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A04187-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026