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| SKU | MA1063 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Human, Mouse, Rabbit, Rat |
| Clonality | Monoclonal |
| Clone Number | MY-32 |
| Host | Mouse |
| Ig Isotype | Mouse IgG1 |
| Applications | IHC, WB |
Overview
| Product Name | Anti-Myosin(Skeletal, Fast) Antibody (Monoclonal, MY-32) |
|---|---|
| SKU/Catalog Number | MA1063 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for Myosin (Skeletal, Fast), myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult (MYH1; MYH2) detection. Tested with WB, IHC-P in Human;mouse;rat;rabbit. No cross reactivity with other proteins. |
| Cite This Product | Anti-Myosin(Skeletal, Fast) Antibody (Monoclonal, MY-32) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1063) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Form | Lyophilized |
| Immunogen | Rabbit muscle myosin. |
| Reactivity | Human, Mouse, Rabbit, Rat |
Assay Details
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1.5-2μg/ml, Human, mouse, rabbit, rat, By Heat
Western blot, 1-2μg/ml, Human, mouse, rabbit, rat
Immunohistochemistry(Paraffin-embedded Section), 1.5-2μg/ml, Human, mouse, rabbit, rat, By Heat
Western blot, 1-2μg/ml, Human, mouse, rabbit, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).Images And Assay Conditions
Anti-Myosin(Skeletal, Fast) antibody (monoclonal), MA1063, Western blotting
WB: Rat Skeletal Muscle Tissue Lysate
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | I6L963 |
|---|---|
| Gene Name | MYH1; MYH2 |
| Protein Name | myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult |
| Alternative Names | Myh2 protein ;Myh2 ; |
| Subcellular Localization | MYH1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|Myh1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|MYH2: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils. |
| Molecular Weight | 183084 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | MYH1: Muscle contraction.|MYH2: Muscle contraction. Required for cytoskeletonorganization (By similarity). |
|---|---|
| Research Areas | Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton / Ecm, Motor Proteins, Myosin, Signal Transduction *You can search these to find other products in these research areas. |
| Background | Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. The light chains are of 2 distinct types: the phosphorylatable, regulatory, or MLC2 type, and the nonphosphorylatable, alkali, or MLC1 and MLC3 types(MYL1 according to the HGM symbols). Skeletal Myosin(Fast),, also known as MYL1, mapped to the region 2q32.1-qter. |
Other Recommended Resources
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Publications
Nie Yw, Ding Xb, Ge Xg, Fan Hl, Liu Zw, Guo H. Cell Biol Int. 2013 Sep;37(9):972-6. Doi: 10.1002/Cbin.10124. Epub 2013 May 23. Enhanced Expression Of Myf5 And Myod1 In Fibroblast Cells Via The Forced Expression Of Bos Taurus Myf5.
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is myosin light chain 3 antibody