Anti-Myosin(Skeletal, Fast) Antibody (Monoclonal, MY-32)

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Datasheet

Protocols

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SKU MA1063
Size 100μg/vial
Reactivity Human, Mouse, Rabbit, Rat
Clonality Monoclonal
Clone Number MY-32
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Myosin(Skeletal, Fast) Antibody (Monoclonal, MY-32)
SKU/Catalog Number MA1063
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Myosin (Skeletal, Fast), myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult (MYH1; MYH2) detection. Tested with WB, IHC-P in Human;mouse;rat;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Myosin(Skeletal, Fast) Antibody (Monoclonal, MY-32) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1063)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Rabbit muscle myosin.
Reactivity Human, Mouse, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 1.5-2μg/ml, Human, mouse, rabbit, rat, By Heat
Western blot, 1-2μg/ml, Human, mouse, rabbit, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1063 1 WB anti myosin skeletal fast antibody monoclonal my 32.jpg

Anti-Myosin(Skeletal, Fast) antibody (monoclonal), MA1063, Western blotting
WB: Rat Skeletal Muscle Tissue Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id I6L963
Gene Name MYH1; MYH2
Protein Name myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult
Alternative Names Myh2 protein ;Myh2 ;
Subcellular Localization MYH1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|Myh1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|MYH2: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.
Molecular Weight 183084 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function MYH1: Muscle contraction.|MYH2: Muscle contraction. Required for cytoskeletonorganization (By similarity).
Research Areas Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton / Ecm, Motor Proteins, Myosin, Signal Transduction

*You can search these to find other products in these research areas.
Background Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. The light chains are of 2 distinct types: the phosphorylatable, regulatory, or MLC2 type, and the nonphosphorylatable, alkali, or MLC1 and MLC3 types(MYL1 according to the HGM symbols). Skeletal Myosin(Fast),, also known as MYL1, mapped to the region 2q32.1-qter.

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Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

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Troubleshooting

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Publications

Nie Yw, Ding Xb, Ge Xg, Fan Hl, Liu Zw, Guo H. Cell Biol Int. 2013 Sep;37(9):972-6. Doi: 10.1002/Cbin.10124. Epub 2013 May 23. Enhanced Expression Of Myf5 And Myod1 In Fibroblast Cells Via The Forced Expression Of Bos Taurus Myf5.
PubMed ID23640900

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is myosin light chain 3 antibody